SINGLE MOLECULES ON CELL MEMBRANES

荧光相关光谱学非常适合于 单个荧光分子的检测和分析 单个罗丹明B的荧光爆发的时间痕迹 分子通过激发体积扩散。FCS使用 双光子激发提供了数学上的优势 清晰的焦点体积的三维几何形状。在.期间 单个分子通过激发扩散的每个事件 体积，可以分析的荧光猝发相对于快速 具有很高统计精度的动力学过程。我们调查了 FCS和Burst分析细胞膜中的单个OD-TMR分子 使用多通道标量。

Fluorescence correlation spectroscopy is ideally suited for detection and analysis of single fluorescent molecules, as exemplified by time traces of fluorescence bursts of individual rhodamine B molecules diffusing through the excitation volume. FCS using two-photon excitation provides the advantage of a mathematically well-defined three-dimensional geometry of the focal volume. During each event of a single molecule diffusing through the excitation volume, the burst of fluorescence can be analyzed in respect to fast kinetic processes with high statistical accuracy. We investigated single OD-TMR molecules in cell membranes by FCS and burst analysis with a multichannel scalar.