STRUCTURE & FUNCTION OF HELICASES

大肠杆菌DNAB解旋酶，似乎是一个六角体，需要三磷酸腺苷， 像大多数解旋酶一样。一种含有半胱氨酸的突变体已经在 推测的ATP结合位点，希望能标记Au 复合体中的ATP-水解点。然而，早期的Au标签尝试 似乎使解旋酶分解成单体。蒙斯到访 实验室花了三个月的时间来研究解旋酶的金标记。一 方法1 S*制备Au-三磷酸腺苷的共价偶联。一 可以使用具有较小Au核的十年金，或者使用 纳米金，这是更明显的。人们还可以标记为ATP、ADP或 非水解性衍生物。这项工作正在进行中。

The E coli DnaB helicase, appears to be a hexamer and requm ATP, like most helicases. A mutant had been created with a cysteine in the putative ATP-binding site in the hopes of Au-labeling the ATP-hydrolysis site in the complex. However, early Au-labeling tries seemed to make the helicase fall apart into monomers. Montse visited the lab for three months to work on Au-labeling of the helicase. One promi ig approach 1 s* to make a covalent conjugate of Au-ATP. One could use either the undecagold, which has a smaller Au core, or the Nanogold, which is more visible. One could also label ATP, ADP, or non hydrolyzable derivatives. This work is stiff in progress.