PHTHALOCYANINE PHOTOSENSITIZER ON PTK2 RAT KANGAROO EPITHELIAL KIDNEY CELLS

本项目的目的是测量荧光量子 产率，荧光寿命，三重态量子产率 寿命为15光敏剂，并将这些 体外光灭活比较测量 这15种光敏剂对PTK 2袋鼠大鼠的效率 肾上皮细胞 这15种光敏剂是： 酞菁（PC）及其二磺酸盐和四磺酸盐（PcDs，PcTs） 衍生物，Pc、PcDs和PcTs的锌金属化络合物; 氯铝酞菁及其四磺酸盐（CIAIPc， CIAIPcTs）;萘啶（Npc），其锌金属化络合物 脱镁叶绿酸甲酯a（Mppb）;单-L-乙酰基二氢卟酚e6 （MACE）;锡代紫嘌呤（SnEt）;血卟啉-IX（Hp），和 Photofrin（PII），HpD的浓缩形式。 光物理 测量将在磷酸盐缓冲盐水（PBS）中进行，其中 可能的，或在PBS-Cremophor中用于二氢卟酚衍生物。 光敏剂将被引入PTK 2细胞24 小时，暴露于来自Nd-YAG的适当波长的光 激光，和光杀伤效率的每一个复杂的评估，利用 台盼蓝细胞活性测试 这项实验旨在 提供了一个预测光杀伤效率的模型， 光敏剂基于它们的物理化学性质。

The aim of this project is to measure the fluorescence quantum yields, fluorescence lifetime, triplet quantum yields an triplet lifetime for fifteen photosensitizers, and correlate these measurements with the comparative in vitro photoinactivation efficiency of these fifteen photosensitizers on PTK2 kangaroo rat epithelial kidney cells. The fifteen photosensitizers are: phthalocyanine (PC), its di and tetrasulfonate (PcDs, PcTs) derivatives, zinc metallated complex of Pc, PcDs, and PcTs; chloroaluminum phthalocyanine and its tetrasulfonate (CIAIPc, CIAIPcTs); naphthalocyanine (Npc), its zinc metallated complex (ZnNPc); methyl pheophorbide a (Mppb); mono-L-aspartyl chlorin e6 (MACE); tin etiopurpurine (SnEt); hematoporphyrin-IX (Hp), and Photofrin (Pll), a concentrated form of HpD. Photophysical measurements will take place in phosphate buffered saline (PBS) where possible, or in PBS-Cremophor for the chlorin derivatives. Photosensitizers will be introduced to PTK2 cells for twenty-four hours, exposed to light at the appropriate wavelength from a Nd-YAG laser, and the photokill efficiency of each complex assessed utilizing the trypan blue test for cell viability. This experiment seeks to provide a model for predicting the photokill efficiency of photosensitizers based on their photophysical properties.