CDC2-RELATED KINASES AND THE CONTROL OF CELL CYCLE

描述(改编自调查人员摘要)：在过去两年中 多年来，这些调查人员已经了解到了 帮助理解这些依赖于细胞周期蛋白的激酶是如何 认识到他们的底物以及他们可能如何开发技术方法 以允许快速识别细胞周期调节的底物 激活剂。研究人员开发了两种技术来筛查 CDK2激酶的新底物，控制最后步骤的酶 G1和DNA合成的早期步骤。他们已经确定了六个新的 到目前为止潜在的细胞周期蛋白E/CDK2底物。其中两种新的底物具有 具有足够的细节特征，足以相信它们在体内 底物。在本申请书中，建议继续审查 Cyclin/CDK复合体如何识别它们的底物，以扩展 验证底物筛选方法，以将这些筛选扩展到 寻找一组其他CDK家族成员的底物，并 通过这些屏幕研究新底物的功能。

DESCRIPTION (adapted from investigator's abstract): During the last two years these investigators have learned several key properties of the cyclin-dependent kinases that have helped understand how these kinases recognize their substrates and how they might develop technical approaches to allow rapid identification of substrates for the cell-cycle-regulating kinases. The investigators have developed two techniques for screening of new substrates of the CDK2 kinases, enzymes that control the last steps of G1 and the early steps of DNA synthesis. They have identified six new potential cyclin E/CDK2 substrates to date. Two of the new substrates have been characterized in sufficient detail to believe that they are in vivo substrates. In this application, it is proposed to continue examination of how cyclin/CDK complexes recognize their substrates, to expand the validation of the substrate screening methods, to extend these screens to look for substrates for a limited set of other CDK family members, and to study the function of the new substrates through these screens.