MULTIFREQUENCY AND HIGH FREQUENCY EPR OF SPIN-PROBES

描述（改编自申请人的摘要）： 本修订后的伊利诺伊州EPR研究中心（IERC）的更新建议， NIH研究资源中心是为了帮助有价值的研究，特别是 生物医学研究，通过开发，建立和提供特殊的 与电子顺磁共振相关的资源、方法和服务 光谱学（EPR），特别强调多频和 高频EPR IERC的具体目标是提供 科学，特别是生物医学研究界，并协助 将EPR应用于他们的研究问题。其中包括：（1）合作 和需要使用EPR设施的服务（常规和特殊 国家的艺术）和专业知识，（2）技术研究和开发以及 作为仪器、方法和应用的演示，（3）培训 有用的技术，和（4）广泛的国家和世界范围内的广泛传播， 可能对EPR活性生物医学有用的各种信息 科学界。IERC的特殊技术包括： 多频EPR和双磁共振从极低（无线电） 频率）DNP到高（毫米波接近远红外）光谱仪频率- 有许多与健康相关的应用，例如， 用于诊断放射学和确定有关结构和动力学的细节 在蛋白质和DNA中。第一优先技术研发 下一个资助期的重点将是高频率的研究， 多频EPR的结构和动力学的蛋白质和DNA， 自旋标记，特别是定点诱变和定点自旋 标签。第二优先技术研究和开发重点将 将EPR技术发展和应用于金属离子的结构和动力学研究， 与生物学相关的激进分子八、技术研发 项目详细说明了集中于这些优先事项的具体应用， 将增加一些额外的仪器来支持它们。这些部分 修订和扩大，以回应以前的审查员的关注。 更新期间，广泛宣传、培训、服务 将开展活动。这些活动包括举办一些国家讲习班， 高频EPR方法，出版大量的通讯服务 对EPR感兴趣的生物医学和其他研究团体的成员， 通过维持一个IERC，支持一个庞大的科学家网络 数据库和因特网信息网址，并作为信息交换所， 交换信息。

DESCRIPTION (Adapted from the applicant's abstract): The broad objective of this revised proposal for renewal of the Illinois EPR Research Center (IERC) as an NIH Research Resource Center is to aid meritorious research, especially biomedical research, by developing, establishing, and providing special resources, methodology, and services related to electron paramagnetic resonance spectroscopy (EPR), with particular emphasis on multifrequency and high-frequency EPR. The specific aims of the IERC are to provide the scientific, especially biomedical, research community with assistance in applications of EPR to their research problems. This includes (1) collaboration and service requiring access to EPR facilities (both routine and special state-of-the art) and expertise, (2) technical research and development as well as demonstration of instrumentation, methods, and applications, (3) training in useful techniques, and (4) extensive national worldwide dissemination of a wide variety of information that might be useful to the EPR-active biomedical scientific community. Special techniques of the IERC include these: multifrequency EPR and double magnetic resonance from very low (radio frequency) DNP to high (mm-wave approaching far-IR) spectrometer frequencies- with many health-related applications, for example, improving pharmaceuticals for diagnostic radiology and determining details about structure and dynamics in proteins and DNA. The first-priority Technological Research and Development focus in the coming grant period will be the study by high frequency and multiple-frequency EPR of structure and dynamics in proteins and DNA through spin labeling, especially site-directed mutagenesis and site-directed spin labeling. The second-priority Technological Research and Development focus will be EPR development and applications to structure and dynamics of metal ions and radicals of biological relevance. Eight Technical Research and Development projects detail specific applications concentrating on these priorities and some additional instrumentation will be added to support them. These sections are revised and expanded in response to previous reviewers' concerns. During the renewal period, extensive dissemination, training, and service activities will be pursued. These include conducting some national workshops on high-frequency EPR methods, publishing a substantial Newsletter to serve members of the biomedical and other research community interested in EPR, supporting a large network of scientists through maintenance of an IERC database and Internet information site, and serving a clearing house for the exchange of information.