STRUCTURAL ANALYSIS OF THE GENOME OF RESPIRATORY SYNCYTIAL VIRUS

呼吸道合胞病毒(RSV)是一种囊膜RNA病毒，是全球儿童呼吸道疾病最重要的病原体。它目前缺乏获得许可的疫苗。RSV是单毒目副粘病毒家族的一员，是一种非节段性负链RNA病毒，是该组病毒中最复杂的成员。这一大群病毒包括重要的病原体，如麻疹、腮腺炎、狂犬病和埃博拉病毒。了解RSV基因组的组织和表达将有助于指导将减毒重组病毒作为疫苗的努力，这是本实验室相关项目的主要目标。我们以前对RSV的15,222个核苷酸的基因组进行了测序，定位了它的基因和转录信号，并分析了它的基因表达机制。我们目前正在对基因组和抗基因组(复制中间)RNA的3端的启动子和封装信号进行详细的突变和功能分析。-病毒、疫苗、减毒活病毒疫苗、儿科、传染病、呼吸道疾病、重组DNA

Respiratory syncytial virus (RSV) is an enveloped RNA virus that is the most important viral agent of pediatric respiratory tract disease worldwide. It currently lacks a licensed vaccine. RSV is a member of the paramyxovirus family of Order Mononegavirales, the nonsegmented negative strand RNA viruses, and RSV is the most complex member of the group. This large group of viruses includes important pathogens such as measles, mumps, rabies and Ebola viruses. Knowledge of the organization and expression of the RSV genome would help guide efforts to make attenuated recombinant viruses as vaccines, which is the major goal of related projects in this laboratory. We previously sequenced the 15,222-nucleotide genome of RSV, mapped its genes and its transcription signals, and analyzed its mechanism of gene expression. We currently are performing detailed mutagenic and functional analysis of the promoters and encapsidation signals at the 3 end of genomic and antigenomic (replicative intermediate) RNA. - Virus, vaccine, live- attenuated viral vaccine, pediatrics, infectious disease, respiratory tract disease, recombinant DNA