CORE-- TISSUE CULTURE

组织培养核心的主要目标是提供具有良好特性的 原代培养神经细胞、星形胶质细胞和嗜铬细胞瘤细胞 4个研究项目中的3个。萨默斯博士，核心主任，已经 在过去的14年里，对所有这些文化有丰富的经验 单元类型。原代大鼠神经元培养已在核心中被记录 由大约90%的神经元和10%的胶质细胞(星形胶质细胞、小胶质细胞)组成 神经丝抗体免疫荧光染色的证据 (Ne-14)。原代培养的大鼠星形胶质细胞几乎100%是胶质细胞， GFAP染色和PC12细胞来源于商业来源。这些 细胞将被用于各种研究，这些研究对于实现 项目1、2、3的具体目标。

The major aim of the Tissue Culture Core is to provide well characterized cultures of primary neuronal, astroglial and pheochromocytoma cells for three of the 4 research projects. Dr. Summers, the Core Director, has had extensive experience over the past 14 years with cultures of all of these cell types. Primary rat neuronal cultures has been documented in the Core to consist of about 90% neurons and 10% glia (astrocytes, microglia) as evidence form immunofluorescence staining with a neurofilament antibody (NE-14). Primary rat astroglia cultures are nearly 100% glia as evident by GFAP staining and PC12 cell are obtained from a commercial source. These cells will be used in a variety of studies that are essential for achieving the specific aims of Project 1, 2 and 3.