QUANTITATIVE DETECTION OF CYTOMEGALOVIRUS BY USING A COMMERCIALLY DEVELOPED RESEA

巨细胞病毒（CMV）可能是破坏性的病人谁经历了异基因骨髓移植。快速检测这些患者的巨细胞病毒感染可以挽救生命，从而可以及时给予抗巨细胞病毒药物。临床上还需要定量测量临床标本中存在的CMV，以监测治疗和预测耐药变体的出现。商业供应商开发了一种研究检测方法，允许使用定量聚合酶链反应（PCR）来测量从全血中提取的CMV DNA。我们将通过使用美国医学实验室收集的外周血白细胞评价该PCR检测，并将结果与CMV抗原血症定量检测（在美国医学实验室进行）进行比较，后者检测多形核白细胞和单核细胞中CMV pp65早期结构蛋白的存在。

Cytomegalovirus (CMV) can be devastating in the patient who has undergone an allogeneic bone marrow transplant. Rapid detection of CMV infection in these patients can be life-saving, allowing the prompt administration of anti-CMV drugs. There is also a clinical need for a quantitative measure of the CMV present in the clinical speci-men to allow for monitoring therapy and predicting emergence of drug-resistant variants. A research assay has been developed by a commercial vendor allowing the use of quanti-tative polymerase chain reaction (PCR) to measure the CMV DNA extracted from whole blood. We will evaluate this PCR assay by using peripheral blood leukocytes collected by American Medical Laboratories and compare the results with the CMV antigenemia quan-titative assay (performed at American Medical Laboratories) that detects the presence of CMV pp65 early structural protein in polymorphonuclear leukocytes and monocytes.