Reagentless Biochip for Inorganic & Organic Contaminants

描述(由申请人提供)： 在NIEHS SBIR下，Biopraxis建议开发一种高通量 用于筛选大种群和大量环境的涂鸦 各种化学污染物的样本，包括重金属、多氯联苯、 杀虫剂、抗生素残留和自然产生的毒素。涂鸦虫是 一种新的无试剂生物芯片技术正在开发中，用于检测 (CBW)药剂和爆炸物。第一阶段将展示Doodlebug可以检测到低- 分子量污染物；单独识别交叉反应样品 成分；同时分析无机物和有机物；并检测这些 复杂的真实世界样本中的目标，而不需要任何样本准备或 清理步骤。早期的研究已经证明，有能力特别地 确定交叉反应毒素和硝基芳香烃，并已确定 可用于识别交叉反应的二价金属的生物分子 阳离子；在这些研究基础上开发的方法将适用于 NIEHS计划。到第二阶段结束时，一种简单的试纸生物芯片将 在几微升的样本中孵育，然后自动读出，在 秒，使用独特的“特定传感器”。因为传感器可以 分析微米级的生物分子像素，生物芯片将会便宜，而且 由于不需要试剂，而且样本可以非常小，Doodlebug将 要非常节俭。 建议的商业应用： NIESH正在寻找新的工具来筛查大量人群是否接触无机和 有机环保剂。然而，Doodlebug将有许多其他应用程序，如 包括场地特征，废水监测，食品和饮料分析， 和医学诊断学。也就是说，Doodlebug可以通过以下方式帮助防止人类接触 帮助清理现有污染，防止未来污染，并检测污染物 在食品中；并可用于帮助诊断因暴露而引起的疾病。

DESCRIPTION (provided by applicant): Under the NIEHS SBIR, Biopraxis proposes to develop a high-throughput Doodlebug for screening large populations and large numbers of environmental samples for diverse chemical contaminants, including heavy metals, PCBs, pesticides, antibiotic residues, and naturally occurring toxins. Doodlebug is a new, reagentless biochip technology under development for the detection of (CBW) agents and explosives. Phase I will show that Doodlebug can detect low- molecular-weight contaminants; individually identify cross-reactive sample constituents; analyze inorganics and organics simultaneously; and detect these targets in complex real world samples, without any sample preparation or cleanup steps. Earlier studies have demonstrated the ability to specifically identify cross-reactive toxins and nitroaromatics, and have identified biomolecules that can be used to identify cross-reactive divalent metal cations; the methods developed on these studies will be adapted for use on the NIEHS program. By the end of Phase II, a simple, dipstick biochip will be incubated in a few microliters of sample, and then read out automatically, in seconds, using a unique "specific transducer." Because the transducer can analyze micron-sized biomolecule pixels, the biochips will be inexpensive, and because no reagents are required and samples can be very small, Doodlebug will be very economical. PROPOSED COMMERCIAL APPLICATION: NIESH is seeking new tools for screening large populations for exposure to inorganic and organic environmental agents. However, Doodlebug will have many other applications as well, including site characterization, wastewater monitoring, food and beverage analysis, and medical diagnostics. I.e., Doodlebug can be used to help prevent human exposure by helping to clean up existing pollution, preventing future pollution, and detecting contaminants in food products; and can be used to help diagnose illness due to exposure.