CB-Sherlock-Reagentless Biochip for Chemical Agents

描述（由申请人提供）：Biopraxis拟开发一种新的， 无试剂生物芯片技术评估人类对有毒工业品的接触 大规模杀伤性武器或恐怖主义的化学品和化学制剂。这 技术已经被证明能够检测食物中的毒素， 环境样品和环境样品中的爆炸物，无样品 清洁步骤。第一阶段将证明它可以检测非常低的分子量 氰化物、DFP和对硝基苯酚等污染物;单独识别 交叉反应样品成分;分析蒸汽和液体样品; 并检测复杂的“真实的世界”样本中的目标，如尿液和呼吸。 早期的研究已经证明， 交叉反应毒素、爆炸物、二价金属阳离子和病原体; 这些研究开发的方法将适用于CDC项目。 到第二阶段结束时，一个简单的试纸生物芯片将在几个 微升样品或短暂暴露于空气中，然后读出 自动，在几秒钟内，使用一个独特的特定传感器。因为 传感器可以分析微米大小的生物分子像素，生物芯片将是 便宜;并且因为不需要试剂并且样品可以非常 小，这项技术将非常经济。 拟定商业应用： 疾病预防控制中心正在寻求快速，可靠，实地坚固的方法来评估人类暴露于大规模毁灭性武器或恐怖主义。然而，该产品也将有许多其他应用，包括用于国防和安全的生化武器和爆炸物检测;用于污染预防和修复的废水监测和现场表征;用于人类健康和安全的食品和饮料分析和环境监测;以及医疗诊断。

DESCRIPTION (provided by applicant): Biopraxis proposes to develop a new, reagentless biochip technology to assess human exposure to toxic industrial chemicals and chemical agent weapons of mass destruction or terrorism. This technology has already been shown to be capable of detecting toxins in food and environmental samples, and explosives in environmental samples, without sample clean-up steps. Phase I will show that it can detect very low molecular weight contaminants such as cyanide, DFP, and paranitrophenol; individually identify cross-reactive sample constituents; analyze vapor as well as liquid samples; and detect targets in complex "real world" samples, such as urine and breath. Earlier studies have demonstrated the ability to specifically identify cross-reactive toxins, explosives, divalent metal cations, and pathogens; the methods developed on these studies will be adapted for use on the CDC program. By the end of Phase II, a simple dipstick biochip will be incubated in a few microliters of sample or exposed briefly to air, and then read out automatically, in seconds, using a unique specific transducer. Because the transducer can analyze micron-sized biomolecule pixels, the biochips will be inexpensive; and because no reagents are required and samples can be very small, the technology will be very economical. PROPOSED COMMERCIAL APPLICATION: CDC is seeking rapid, reliable, field-rugged methods for assessing human exposure to weapons of mass destruction or terrorism. However, the product will have many other applications as well, including CBW agent and explosives detection for defense and security; wastewater monitoring and site characterization for pollution prevention and remediation; food and beverage analysis and environmental monitoring, for human health and safety; and medical diagnostics.