CB-Sherlock-Reagentless Biochip for Chemical Agents

描述(申请人提供)：Biopraxis建议开发一种新的无试剂生物芯片技术来评估人类对有毒工业的暴露 化学品和化学制剂大规模杀伤性武器或恐怖主义。这 技术已经被证明能够检测食物中的毒素和 环境样品和环境样品中的爆炸物，不带样品 清理步骤。第一阶段将显示它可以检测到非常低的分子量 氰化物、DFP和对硝基苯酚等污染物；单独识别 交叉反应样品成分；分析蒸气和液体样品； 并在复杂的“现实世界”样本中检测目标，如尿液和呼吸。 早期的研究已经证明，有能力专门识别 交叉反应毒素、炸药、二价金属阳离子和病原体； 在这些研究基础上开发的方法将被调整用于疾控中心计划。 到第二阶段结束时，一个简单的试纸生物芯片将在几个 微升样品或短暂暴露在空气中，然后读出 自动，在几秒钟内，使用唯一的特定传感器。因为 传感器可以分析微米级的生物分子像素，生物芯片将被 便宜；因为不需要试剂，样品可以非常 体积小，这项技术将非常经济。 建议的商业应用： 疾控中心正在寻找快速、可靠、实地坚固的方法来评估人类接触大规模杀伤性武器或恐怖主义的情况。然而，该产品还将有许多其他应用，包括用于国防和安全的CBW试剂和爆炸物检测；用于污染预防和修复的废水监测和现场表征；用于人类健康和安全的食品和饮料分析和环境监测；以及医疗诊断。

DESCRIPTION (provided by applicant): Biopraxis proposes to develop a new, reagentless biochip technology to assess human exposure to toxic industrial chemicals and chemical agent weapons of mass destruction or terrorism. This technology has already been shown to be capable of detecting toxins in food and environmental samples, and explosives in environmental samples, without sample clean-up steps. Phase I will show that it can detect very low molecular weight contaminants such as cyanide, DFP, and paranitrophenol; individually identify cross-reactive sample constituents; analyze vapor as well as liquid samples; and detect targets in complex "real world" samples, such as urine and breath. Earlier studies have demonstrated the ability to specifically identify cross-reactive toxins, explosives, divalent metal cations, and pathogens; the methods developed on these studies will be adapted for use on the CDC program. By the end of Phase II, a simple dipstick biochip will be incubated in a few microliters of sample or exposed briefly to air, and then read out automatically, in seconds, using a unique specific transducer. Because the transducer can analyze micron-sized biomolecule pixels, the biochips will be inexpensive; and because no reagents are required and samples can be very small, the technology will be very economical. PROPOSED COMMERCIAL APPLICATION: CDC is seeking rapid, reliable, field-rugged methods for assessing human exposure to weapons of mass destruction or terrorism. However, the product will have many other applications as well, including CBW agent and explosives detection for defense and security; wastewater monitoring and site characterization for pollution prevention and remediation; food and beverage analysis and environmental monitoring, for human health and safety; and medical diagnostics.