CULTURED CILIATED CELLS FOR HIGH THROUGHPUT SCREENING

描述(申请人摘要)：BioTechPlex建议开发一种 哺乳动物、气管/支气管呼吸道上皮细胞系 吞吐量筛选。这是基于生理机能测定的。这些 在二次培养后，细胞将至少有3-6周的寿命。 该项目的成果提供了第一个细胞模型系统 也用于新一代HTS平台的肺部药物发现 作为服务于细胞生理学/药理学研究的特定需求 组织工程学。在这个第一阶段的项目中，BioTechPlex将开发一个 培养呼吸道上皮细胞以使其纤毛 功能保存超过16周，并继代培养这些细胞 在涂有胶原蛋白的水下介质中或使用气-液界面。这个 研究人员将开发一种技术来重新激活休眠的纤毛生成 在这些上皮细胞中再生纤毛的机制 50%的细胞有纤毛，这种情况与 在体内和天然的气管和主要的支气管上皮中发现。同相 BioTechPlex将建造一个旋转生物反应器系统，以生产大型 主要哺乳动物呼吸道纤毛上皮细胞的数量 用于上皮研究和药物筛选的物种。 建议的商业应用： 不可用

DESCRIPTION (Applicant's Abstract): BioTechPlex proposes to develop a mammalian, tracheal/bronchial airway epithelial cell line specifically for high throughput screening. This is based on physiological functional assay. These cells will have a minimum of 3-6 weeks life-span following secondary culture. The outcome of this project provides the first cell model system that can be used for a new generation of HTS platform for pulmonary drug discovery as well as serving the specific needs for cellular physiology/pharmacology research and tissue engineering. In this Phase I project, BioTechPlex will develop a technique to culture the airway epithelial cells such that their ciliary functions are preserved longer than 16 weeks and sub-culture these cells either in collagen-coated submerged medium or using an air-liquid interface. The investigators will develop a technique to reactivate the dormant ciliogenesis mechanisms to regenerate the cilia in these epithelial cells with a minimum of 50 percent of the cells are ciliated, a condition in close resemblance to that found in vivo and in native tracheal and major bronchial epithelia. In Phase II, BioTechPlex will construct a rotating bio-reactor system to produce large quantities of these airway ciliated epithelial cells of the major mammalian species used in epithelial research and drug screening. PROPOSED COMMERCIAL APPLICATION: Not Available