Adipose Stromal Cells for Soft Tissue Bioengineering

描述（来自申请人摘要的逐字）：Zen-Bio，Inc专注于 人脂肪组织的体外分离和培养， 在这个领域的国际声誉。新的医疗需求尚未得到满足， 制造植入脂肪组织的技术。脂肪代谢障碍患者输 皮下脂肪，导致危及生命的糖尿病。产生新的脂肪 组织将简化他们的医疗管理。乳腺癌患者常规 接受横直肌腹部肌肉皮肤手术， 乳房切除术以实现乳房再造。其中许多患者可能 接受成本较低和侵入性较低的“肿块切除术”，但选择不，因为 结果在美学上不可接受。生物工程自体脂肪修复 乳房肿瘤切除术将鼓励更多的此类手术。拟议 实验，Zen-Bio Inc.将获得”原理证明”数据， 人类脂肪组织来源的基质细胞可用于软组织 重建和移植。具体目标我会进一步优化 冷冻保存人脂肪源性基质细胞并维持其 体外分化潜能。具体目标2将决定能力 人脂肪来源的基质细胞在植入时形成脂肪垫 皮下注射免疫缺陷小鼠。这些研究将证明， 我们核心假设的商业可行性，即自体脂肪来源 基质细胞可用于软组织重建 拟议商业应用：不可用

DESCRIPTION (Verbatim from the Applicant's Abstract): Zen-Bio, Inc focuses on the in vitro isolation and culture of human adipose tissues and has an international reputation in this field. There is an unmet medial need for new technologies to create implant adipose tissues. Lipodystrophy patients lose subcutaneous fat, leading to life threatening diabetes. Creating new adipose tissue would simplify their medical management. Breast cancer patient routinely undergo a transverse rectus abdominal musculocutaneous procedure after mastectomy to achieve breast reconstruction. Many of these same patients could undergo a less costly and invasive "lumpectomy" but elect not to since the outcome is aesthetically unacceptable. Bio-engineered autologous fat to repair lumpectomies would encourage more of these procedures. In the proposed experiments, Zen-Bio Inc. will obtain" proof of principle" data demonstrating that human adipose tissue derived stromal cells can be used for soft tissue reconstruction and transplanting. Specific Aim I will further optimize cryopreservation of human adipose-derived stromal cells and maintain their differentiation potential in vitro. Specific Aim 2 will determine the ability of human adipose-derived stromal cells to form a fat pad when implanted subcutaneously in immunodeficient mice. These studies will demonstrate the commercial feasibility of our core hypothesis, that autologous adipose derived stromal cells can be used for soft tissue reconstruction PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE