Hematopoietic Support by Adipose-Derived Stromal Cells
Hematopoietic Support by Adipose-Derived Stromal Cells
批准号:
6435095
负责人:
Jeffrey Martin Gimble
金额:
$10.0万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2001
资助国家:
美国
项目状态:
已结题
起止时间:
2001-06-18 至 2001-12-17
中文摘要
骨髓移植需要大量的多能造血细胞
干细胞取得了成功的临床结果。因此,有一个迫在眉睫的
医学上需要在体外扩增干细胞并加速其
体内植入。这些步骤可能得到产生细胞因子的基质的支持
细胞。Artecel Science的科学家已经开发出专有技术来
从脂肪组织中分离培养人基质细胞,可用于
自体和异体移植。这篇SBIR调查的是假设
脂肪来源的基质细胞将(1)支持造血祖细胞
体外增殖和分化;(2)促进其体内植入。
目标1(阶段I)将证明人类脂肪来源的基质细胞将
支持多向和多向造血祖细胞的增殖
用流式细胞仪和甲基纤维素法进行短期和长期培养。这
这项工作为H阶段研究提供了基础,以确定:(目标3)
体外扩增的造血祖细胞在亚致死剂量照射后的再生
NOD/SCID小鼠;(目标4)脂肪来源的基质细胞加速
亚致死剂量照射的NOD/SCID小鼠的造血干细胞植入。
建议的商业应用:
这项SBIR将开发出适用于所有接受骨移植的患者的技术
骨髓移植。每年有20,000名接受这种疗法的患者
个人花费100,000美元。Artecel Sciences的技术有可能
大大减少了这些程序的临床和经济负担。
英文摘要
Bone marrow transplantation requires large numbers of pluripotent hematopoietic
stem cells to achieve a successful clinical outcome. Therefore, there is a pressing
medical need for methods to expand stem cells ex vivo and accelerate their
engraftment in vivo. These steps may be supported by cytokine-producing stromal
cells. The scientists at Artecel Sciences have developed proprietary techniques to
isolate and culture human stromal cells from adipose tissue that can be used for
autologous and allogeneic transplantation. This SBIR investigates the hypotheses
that adipose derived stromal cells will (1) support hematopoietic progenitor cell
proliferation and differentiation in vitro and (2) accelerate their engraftment in vivo.
Aim 1 (Phase I) will demonstrate that human adipose derived stromal cells will
support proliferation of multipotent and pluripotent hematopoietic progenitors in
short and long term cultures using flow cytometric and methylcellulose assays. This
work provides a foundation for Phase H studies to determine: (Aim 3) The ability of
ex vivo expanded hematopoietic progenitors to repopulate sublethally irradiated
NOD/SCID mice; (Aim 4) The ability of adipose derived stromal cells to accelerate
hematopoietic stem cell engraftment in sublethally irradiated NOD/SCID mice.
PROPOSED COMMERCIAL APPLICATION:
This SBIR will develop technology with application to all patients receiving bone
marrow transplantation. Each year, 20,000+ patients under this therapy at an
individual cost of $100,000. Artecel Sciences technology has the potential to
significantly reduce the clinical and financial burden of these procedures.
期刊论文(1)
专著(0)
科研奖励(0)
会议论文
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批准号:8368987
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Botannical Extract Library Screeninig with Human ADAS Cells
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批准号:7478265
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资助金额:$22.9万
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财政年份:2008
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负责人:Jeffrey Martin Gimble
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依托单位:
LOUISIANA COBRE: OBESITY & DIABETES RES: CELL BIOLOGY CORE
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批准号:7382258
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批准号:6928684
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项目类别:
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资助金额:$10.0万
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财政年份:2005
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负责人:Jeffrey Martin Gimble
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依托单位:
CIRCADIAN MECHANISMS REGULATING OSTEOGENESIS
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批准号:6857660
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项目类别:
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资助金额:$18.03万
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财政年份:2004
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负责人:Jeffrey Martin Gimble
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依托单位:
CIRCADIAN MECHANISMS REGULATING OSTEOGENESIS
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批准号:6954223
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项目类别:
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资助金额:$18.93万
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财政年份:2004
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负责人:Jeffrey Martin Gimble
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依托单位:
Adipose Stromal Cells for Soft Tissue Bioengineering
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批准号:6457542
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项目类别:
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资助金额:$10.11万
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财政年份:2001
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负责人:Jeffrey Martin Gimble
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依托单位:
USE OF ADIPOSE-DERIVED PREADIPOCYTES IN BONE DISOREDERS
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批准号:6294179
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项目类别:
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资助金额:$16.0万
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财政年份:1999
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负责人:Jeffrey Martin Gimble
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依托单位:
USE OF ADIPOSE-DERIVED PREADIPOCYTES IN BONE DISOREDERS
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批准号:6532983
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项目类别:
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资助金额:$7.85万
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财政年份:1999
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负责人:Jeffrey Martin Gimble
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依托单位:
GENE REGULATION IN LYMPHOHEMATOPOIETIC STROMAL CELLS
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批准号:2094021
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项目类别:
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财政年份:1989
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负责人:Jeffrey Martin Gimble
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GENE REGULATION IN LYMPHOHEMOPOIETIC STROMAL CELLS
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项目类别:
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财政年份:1989
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负责人:Jeffrey Martin Gimble
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依托单位:
GENE REGULATION IN LYMPHOHEMATOPOIETIC STROMAL CELLS
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批准号:2376845
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项目类别:
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资助金额:$2.15万
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财政年份:1989
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负责人:Jeffrey Martin Gimble
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依托单位:
GENE REGULATION IN LYMPHOHEMATOPOIETIC STROMAL CELLS
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批准号:2686106
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资助金额:$8.24万
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财政年份:1989
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批准号:2094017
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资助金额:$7.6万
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财政年份:1989
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批准号:2094020
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GENE REGULATION IN LYMPHOHEMOPOIETIC STROMAL CELLS
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依托单位:
海外基金