ENHANCED DNA MICROARRAY PERFORMANCE WITH GAPPED PROBES

该项目的目标是开发一个更强大的系统，用于测序和分析DNA的杂交到微阵列。我们在概念上新颖的方法是基于一种新的探针设计和序列重建算法，该算法已经通过计算建模证明可以在DNA杂交芯片上提供更高的信息密度。人类基因组计划为广泛的生物现象和疾病提供了独特的见解。生物技术部门和生物技术产品的最终用户将推动一个数十亿美元的基于dna的诊断和研究工具市场。预计到2005年，基于DNA探针的诊断测试将成为一个价值60亿美元的市场。该市场的很大一部分将使用DNA微芯片阵列。我们的方法将为医学诊断和从头测序的DNA分析提供一种优越的方法。改进的DNA微阵列将在临床诊断、药物发现表达谱以及从头DNA测序中发挥作用。

The goal of this project is to develop a more powerful and robust system for the sequencing and analysis of DNA by hybridization to microarrays. Our conceptually novel approach is based on a new probe design and sequence reconstruction algorithm which has been demonstrated by computational modeling to provide higher information density on a DNA hybridization chip. The Human Genome Project is providing unique insight into a wide range of biological phenomena and diseases. The biotechnology sector and end users of biotechnology products will drive a multibillion dollarmarket for DNA-based diagnostics and research tools. By the year 2005 it is expected that diagnostic tests based on DNA probes will be a $6 billion market. A significant portion of this market will use DNA microchip arrays. Our method will provide a superior method for analyzing DNA for medical diagnostics and for de novo sequencing. PROPOSED COMMERCIAL Improved DNA microarrays will be useful in clinical diagnostics, drug discovery expression profiling, as well as de novo DNA sequencing.