Wavelength-coded Latex Beads for Bioassay

描述(逐字摘自申请人的摘要)：许多诊断测试和 分析使用微小尺寸的均匀乳胶颗粒或微球作为底物。 或用于免疫学和核酸分析的载体。麦克斯韦传感器公司 (MSI)提出开发一种创新的波长编码量子珠(WCQB) 多分析物分析技术。建议的技术是基于 由浸渍的量子点粒子形成的光学条码微球 乳胶珠或磁珠的外壳。这项技术的新颖性在于 波长编码的珠子识别正在发生的反应 微球表面。每个WCQB可以执行一个测试，因此每个WCQB是一个 分析物分析仪。通过将WCQB的混合物添加到样本中，数百 分析物可以同时、轻松、快速、廉价地进行测试。这个 第一阶段的工作将集中在WCQB合成、光学解码、准确性 评价，结合能力和生物学特性表征。在……里面 第二阶段，我们将优化WCQB和系统性能，并扩展 包括临床免疫分析和核酸分析在内的技术。 建议的商业应用： 乳胶粒子和磁性粒子是固相分析的理想选择，并已被使用 用于放射免疫分析、ELISA、FIA、细胞分离和化学发光免疫分析， 以及在核酸和分子生物学中的各种应用。通过对乳胶进行光学编码 珠子，可以同时测试数百种分析物。

DESCRIPTION (Verbatim from the Applicant's Abstract): Many diagnostic tests and assays use micro-size uniform latex particles, or microspheres, as substrates or supports for immunological and nucleic acid assays. Maxwell Sensors Inc. (MSI) proposes to develop an innovative wavelength coded quantum-bead (WCQB) technology for multiple-analyte assays. The proposed technology is based on optically bar-coded microspheres formed by impregnated quantum dot particles in a shell of latex bead or magnetic bead. The novelty of the technology is that wavelength-coded beads identify which reaction is taking place on the microsphere surface. Each WCQB can perform one test, thus each WCQB is a single analyte analyzer. By adding a mixture of WCQBs to a sample, several hundred analytes can be tested simultaneously, easily, rapidly, and inexpensively. The Phase I work will focus on WCQB synthesis, optical de-coding, accuracy evaluation, and binding ability and biological property characterization. In Phase II, we will optimize WCQB and system performance, and expand the technology to include clinical immunoassays and nucleic acid assays. PROPOSED COMMERCIAL APPLICATION: Latex particles and magnetic particles are ideal for solid phase assays, and have been used for radioimmunoassays, ELISA, FIA, cell separation, and chemiluminescence immunoassays, and various applications in nucleic acids and molecular biology. By optically coding latex beads, several hundred analytes can be tested simultaneously.