REGULATION OF PROGESTERONE BIOSYNTHESIS BY THE CORPUS LUTEUM

黄体合成和分泌类固醇激素 孕酮，它是建立和维持 怀孕了。黄体和孕酮的分泌也 调节女性月经周期的持续时间和动情周期 家养动物。对黄体的处理导致 妇女的避孕药和同步发情周期的方法 家养动物。然而，胚胎死亡率很高(约为 50%的妇女，16%-42%的牲畜)和目前的不良副作用 一些女性的避孕措施规定了管理主体的新方法 对黄体进行检查。多胺调节细胞生长和细胞内 功能。然而，多胺的作用和黄体功能有 没有被研究过。这项拟议的研究将考察 多胺与孕酮的合成和mRNA的表达 编码黄体内的类固醇合成酶。细胞培养 分子生物学技术将被用来完成这些 目的利用绵羊，它被许多研究人员用作 人类和其他家养动物的动物模型。黄体 将在发情周期的第10天从母羊身上获得。黄体组织 将被酶消化以产生单细胞悬浮液。 培养的细胞将被多胺和孕酮分泌处理。 将由RIA测量。培养的细胞将被收获和mRNA 提取出来的。编码类固醇生成酶的mrna将通过 Northern杂交方法。学生将获得全面的 需要接受细胞/组织培养和分子生物学技术方面的培训 在众多科学领域具有竞争力。

The corpus luteum synthesizes and secretes the steroid hormone progesterone which is required for the establishment and maintenance of pregnancy. The corpus luteum and the secretion of progesterone also regulates the duration of menstrual cycles in women and estrous cycles in domestic animals. Manipulation of the corpus luteum has resulted in contraceptives for women and means of synchronizing estrous cycles in domestic animals. Yet the high rate of embryonic mortality (approximately 50% in women, 16-42% in livestock) and undesirable side effects of current contraceptives for some women dictates new methods to govern the corpus luteum be investigated. Polyamines regulate cell growth and intracellular functions. However, the role of polyamines and corpus luteum function has not been studied. The research proposed will examine the role of polyamines and the synthesis of progesterone and expression of mRNA encoding the steroidogenic enzymes within the corpus luteum. Cell culture and molecular biology techniques will be used to accomplish these objectives utilizing ewes, which are used by numerous researchers as animal models for both humans and other domestic animals. Corpora lutea will be obtained from ewes on day 10 of the estrous cycle. Luteal tissue will be enzymatically digested to yield single cell suspensions. Cultured cells will be treated with polyamines and progesterone secretion will be measured by RIA. Cultured cells will be harvested and mRNA extracted. mRNA encoding the steroidogenic enzymes will be analyzed by Northern blot hybridization methods. Students will receive comprehensive training in cell/tissue culture and molecular biology techniques required to be competitive in numerous scientific fields.