IN UTERO PROTEINASE ACTIVITY DURING HAMSTER EMBRYO ZONA ESCAPE TIME WINDOW

目前，体内透明带逃逸的机制尚不清楚。 理解，以及促成体外“孵化”的因素 来自透明带。本研究的总体目标是 了解体内小带形成的原因 通过研究冲洗液中酶活性的存在来逃避 与天区逃逸时间窗口重合(Gonzales和 Bavister，1995)在怀孕和假孕仓鼠中。 在卵带形成过程中从子宫中收集胚胎和潮水 逃逸时间窗将被用来比较 卵/胚胎，它们在生殖道中的位置，以及 孕妇、假孕妇女和非妊娠妇女体内酶活性的存在 仓鼠怀孕的子宫。因此，这一次的目标是 建议的研究是：1)确定是否存在子宫酶 可能导致体内带状带溶解的活动 与小带逃逸时间窗相吻合 假孕仓鼠，2)检验假设没有 未怀孕的骑行仓鼠雌鼠的卵泡溶解活性 确定透明带体外溶血活性是否与透明带不同 体内的溶解活性。在这项研究中，每个人的贡献 参与透明带逃逸的参与者(透明带、胚胎 和子宫上皮)进行检查，以实现更完整的 对宫内透明带丢失机制的理解。本研究 也将为进一步研究阐明1)提供基础 子宫相关酶活性的调节作用 关键的植入前阶段，以及2)可能的胚胎-母体 对话。

At present, the mechanism of zona escape in vivo is poorly understood, as are the factors contributing to in vitro "hatching" from the zona pellucida. The overall goal of the present study is to obtain an understanding of the contributing factors of in vivo zona escape by studying the presence of enzyme activity in flushates that are coincident with the zona escape time window (Gonzales and Bavister, 1995) in both pregnant and pseudopregnant hamsters. Collections of embryos and flushates from the uterus during the zona escape time window will be used to compare the morphology of eggs/embryos, their location in the reproductive tract, and the presence of enzymatic activity in pregnant, pseudopregnant, and non- pregnant uteri of hamsters. Therefore, the objectives of this proposed study are: 1) to determine if there is a uterine enzyme activity that is potentially responsible for zona lysis in vivo that is coincident with the zona escape time window in both pregnant and pseudopregnant hamsters, 2) to test the hypothesis that there is no zona lytic activity in non-pregnant cycling hamster females and 3) to determine if zona lytic activity is different in vitro than the zona lytic activity in vivo. In this study the contribution of each participants involved in zona escape (the zona pellucida, the embryo and the uterine epithelium) is examined to achieve a more complete understanding of the mechanism of zona loss in utero. This study will also provide a foundation for further research to elucidate 1) regulatory effect of uterine enzyme activity associated with the critical preimplantation period, and 2) possible embryo-maternal dialogue.