CORE--ANIMAL EXPERIMENTATION RESOURCE

产品描述：对生物样品中存在的各种自分泌、旁分泌和神经分泌因子的作用的生物学和临床研究需要准确可靠地测量这些产物的能力。 同样，药物作用的评估需要测量组织和液体提取物中的这些药物及其代谢物，以确定动力学。 这些具体目标构成了分析实验室主要职能的基本原理。 该设施自1989年以来一直存在。 这是一个套房，包括一个600平方米。 英尺 化学实验室，200平方米。 英尺 4 ℃冷藏室，2个设备室，其中包含-70 ℃和-30 ℃冷冻机、旋转真空/冻干机以及伽马和闪烁计数器。该装置完全能够偶联抗原，产生多克隆兔抗体，偶联碘化配体，表征抗体和测定。 此外，它还拥有专门用于鉴定柱免疫反应性的HPLC和用于药物的气相色谱。该实验室具有多种肽（脑啡肽、NPY、速激肽、甘丙肽、蛙皮素、CGRP）、胺和代谢物（儿茶酚胺和吲哚胺）、氨基酸、嘌呤（腺苷、ATP/ADP/AMP）、前列腺素（PGE 1/2; LTB 4/C4/D4）和细胞因子（TNF、IL 1）的在线检测。除了这些激素，各种药物及其代谢物也需要分析。 这些药物包括阿片类药物（吗啡、哌替啶、芬太尼及其衍生物、美沙酮、可待因）、局部麻醉剂（利多卡因、布比卡因、美西律）、注射麻醉剂（丙泊酚、咪达唑仑、巴比妥类药物）和乙醇。 该设施配备了Windows NT工作站和（安全的）校园范围内的E以太网启用的结果分发的数据管理。

Description: (Applicant's Description) Biological and clinical research on the effects of various autocrine, paracrine and neurocrine factors present in biological samples require the ability to accurately and reliably measure these products. Similarly, the assessment of drug action necessitates the measurement of these agents and their metabolites in tissue and fluid extracts to permit definition of kinetics. These specific aims constitute the rationale for the primary function of the Analytical Laboratory. This facility has been in existence since 1989. It is a suite consisting of a 600 sq. ft. chemistry laboratory, a 200 sq. ft. 4 degree C cold room, 2 equipment rooms, which contain -70 and -30 degree C freezers, spin vac/lyophilizer, and gamma and scintillation counters. The unit is fully capable of coupling antigens, creating polyclonal rabbit antibodies, coupling of the iodinated ligand, characterization of the antibody and the assay. In addition, it has dedicated HPLCs for identification of column immunoreactivity and gas chromatography for drugs. The laboratory has on-line assays for a variety of peptides (enkephalins, NPY, tachykinins, galanin, bombesin, CGRP), amines and metabolites (catecholamines and indolamines), amino acids, purines (adenosine, ATP/ADP/AMP), prostanoids (PGE1/2; LTB4/C4/D4), and cytokines (TNF, IL1). In addition to these hormones, a variety of drugs and their metabolites are also subject to analysis. These include opiates (morphine, meperidine, fentanyl and derivatives, methadone, codeine), local anesthetics (lidocaine, bupivacaine, mexiletine) injectable anesthetic (propofol, midazolam, barbiturates) and ethanol. The facility is fully equipped for data management with a Windows NT workstation and (secured) campus-wide E thernet-enabled results distribution.