BAA-APPLICATION OF HLA DATA TO DEVELOP & IMPROVE VACCINE

免疫T淋巴细胞对外来抗原的识别需要呈递短的蛋白质片段，称为肽，其与作为T细胞受体配体的主要组织相容性复合物（MHC）分子结合。在对感染或疫苗接种的免疫应答过程中，存在数千种源自细胞和病原体分子的肽，但其中只有一小部分被表征。目前的项目旨在非常详细地定义由特定的人类MHC I类分子（HLA-A，-B和-C）呈递的肽。 肽将从人免疫缺陷病毒-1（HIV-1）感染前后的人细胞系衍生的纯化HLA分子中提取。将使用色谱法和质谱法生成比较肽图，以分离和测序释放的肽。这种方法将提供广泛的信息，潜在的目标表位可供HLA限制性人类T细胞对艾滋病毒的反应。 一个可搜索的T细胞配体数据库将被开发为一个全面的信息来源的肽提出的HLA分子。

Recognition of foreign antigens by immune T lymphocytes requires the presentation of short protein segments, termed peptides, bound to major histocompatability complex (MHC) molecules that serve as ligands for T cell receptors. Thousands of peptides, derived from both cellular and pathogen molecules, are presented during an immune response to infection or vaccination, but only a small proportion of these have been characterized. The current project seeks to define in great detail the peptides presented by specific human MHC class I molecules (HLA-A, -B, and ~C). Peptides will be extracted from purified HLA molecules derived from human cell lines both before and after infection by human immunodeficiency virus-1 (HIV-1). Comparative peptide maps will be generated using chromatography and mass spectrometry to separate and sequence the released peptides. This approach will provide extensive information on the potential target epitopes available to HLA-restricted human T cells responding to HIV. A searchable T cell ligand database will be developed as a comprehensive source of information on peptides presented by HLA molecules.