Crystallization Tools for Structural Genomics

说明：申请人？s摘要）一种整合的自动化蛋白质 结晶系统RoboHTC（Robotic High-Put Crystallization）将 开发，包括软件和硬件工具，适用于 大规模蛋白质晶体学项目，如结构基因组学。 这些工具也将高度模块化，允许个人家庭实验室 根据需要使用系统的一个或多个组件。这些工具 将解决目前的问题，在结晶，限速步骤， 蛋白质晶体学，在分离和纯化目标之后 蛋白我们还将把这些工具与基于Web的Java applet集成起来 让研究人员可以定制结晶溶液。这将 使研究人员不必自己制作结晶屏幕， 这是目前蛋白质结晶中最乏味的任务之一。 整个晶体学社区将受益于拟议的 research.作为我们结晶数据库进一步发展的一部分 软件，我们将实现的功能，使研究人员可以轻松地转移 蛋白质数据库的结晶数据。这些公开 可访问的结晶数据可以指导其他研究人员 结晶的努力。随着蛋白质晶体学越来越成为 生物学研究的一个重要方面，所提出的工具将使蛋白质 晶体结构测定是任何生物学研究中较为常规的一部分 程序. 拟定商业应用： 不可用

DESCCRIPTION: Applicant?s abstract) An integrated, automated protein crystallization system,RoboHTC (Robotic High-Throughput Crystallization), will be developed, consisting of software and hardware tools suitable for large-scale protein crystallography projects, such as structural genomics. These tools will also be highly modular, allowing individual home laboratories to use one or more components of the system as their needs demand. These tools will address current problems in crystallization, the rate-limiting step in protein crystallography, following isolation and purification of the target protein. We will also be integrating these tools with a Web-based Java applet to allow researchers to order custom crystallization solutions. This will alleviate researchers from having to make their own crystallization screens, which is presently one of the most tedious tasks in protein crystallization. The entire crystallographic community stands to benefit from the proposed research. As part of the further development of our crystallization database software, we will implement features to allow researchers to easily transfer crystallization data to and from the Protein Data Bank. These publicly accessible crystallization data can then guide other researchers in their crystallization efforts. As protein crystallography becomes an increasingly important aspect of biological research, the proposed tools will make protein crystal structure determination a more routine part of any biological research program. PROPOSED COMMERCIAL APPLICATION: Not Available