Core--Molecular neurobiology

Core B旨在为CNS和外周中的神经解剖学、神经化学、神经递质受体以及原位杂交研究提供集中资源。为分子药理学、总RNA分离、溶液杂交-核酸酶保护测定、PCR测定和狭缝印迹RNA分析、Western印迹分析以及Tk肽、BK和细胞因子测定提供集中资源。核心主任将负责监督核心的运作;共同调查员和顾问将负责培训调查员、研究助理和研究技术员。通过提供中央设施，该核心将促进解剖学、神经化学和分子药理学方法的标准化，并确保数据分析的统一标准。作为一项额外服务，该中心将向研究人员提供关于使用药理学工具操纵生理系统的咨询意见，并提供一个生物活性化合物中央储存库（药物库），供研究人员个人使用。这项服务将消除生物活性剂的重复订购，并确保适当的受体药理学方法，或购买必要的设备和用品。

Core B is designed to provide a centralized resource for neuroanatomical, neurochemical, neurotransmitter receptors in the CNS and in the periphery, and for in situ hybridization studies. To provide a centralized resource for molecular pharmacology, isolation of the total RNA, solution hybridization-nuclease protection assays, PCR assays and slot blot RNA analysis, Western blot analysis, and assays for measurements of Tk peptides, BK and cytokines. The core director will be responsible for overseeing operation of the core; co-investigators and consultants will be available to train investigators, research associates and research technicians. By providing a central facility, the core will facilitate standardization of anatomical, neurochemical and molecular pharmacological methods and assure uniform criteria for data analysis. As an additional service, the core will advise investigators concerning the use of pharmacological tools to manipulate physiological systems and provide a central repository of bioactive compounds (drug library) for the use of individual investigators. This service will eliminate duplicate ordering of bioactive agents and assure proper receptor pharmacological methods, or purchase of necessary equipments and supplies.