HIGH EFFICIENCY MAPPING OF ALCOHOL SENSITIVITY GENES

我们建议从近交系长睡眠(ILS)和近交系短睡眠(ISS)品系中构建大量重组自交系(RIS)，并对8个与酒精作用有关的性状进行遗传作图。这一新生成的RI序列将用于数十种不同的行为分析，而只需要执行一组基因类型评估。从单一的基因型评估将流动作图结果适用于在亲本菌株(ILS和ISS)或在LS和SS选择的品系中评估的无数不同的表型。主要的缺点是。与RI相关的品系(在QTL鉴定和作图中缺乏分辨率)是通过使用如此大的品系集来克服的。由科罗拉多大学和行为遗传学研究所的内部资源资助的RI建设已经开始，我们建议将这些RI带到F22，这将允许超过98%的固定。在这个项目完成后，我们将创建一个电子数据库，使所有酒精研究人员能够轻松获取我们的所有结果。我们还将开始作出安排，将这些鼠标分发给希望使用它们的搜索者。

We propose to complete the construction of a large number of recombinant inbred strains (RIs) from the inbred Long Sleep (ILS) and the inbred Short Sleep (ISS) strains and to map genetically eight traits involved in alcohol action. This newly generate RI series will be useful in dozens of different behavioral analyses, while only one set of genotypic assessment swill need to be performed. From the single assessment of genotype will flow mapping results applicable to a myriad of various phenotypes that have been assessed in the parental strains (ILS and ISS) or in the LS and SS selected lines. The major disadvantages. associated with RI strains (lack of power in QTL identification and lack of resolution in mapping) are overcome through the use of such a large set of strains. The RI construction has already begun, funded by internal resources of the University of Colorado and the Institute for Behavioral Genetics and we propose to carry these RIs to the F22, which will allow greater than 98% fixation. At the completion of this project we will create an electronic data base allowing all alcohol researchers easy access to all of our results. We will also begin making arrangements to distribute these mice to searchers who wish to use them.