EFFECTS OF COCAINE IN FETAL MONKEY BRAIN

尽管进行了密集的研究，但与 对艾滋病毒感染的保护性免疫仍然知之甚少。 减毒活疫苗诱导的细胞免疫应答分析 SIV提供了一个理想的研究环境，在其中定义特定的 可能在调解保护方面发挥作用的机制。恒河猴 接种SIV239？NEF或SIV239？3疫苗的猕猴发育旺盛 SIV特异性细胞毒T淋巴细胞反应和CD8+淋巴细胞 感染了减毒活SIV毒株的动物能够抑制 SIV复制通过MHC受限机制和 生产可溶的因子。SIV特异性的前瞻性分析 不同减毒活疫苗感染动物的CTL反应 菌株提示诱导强烈的SIV特异性免疫 反应与对阴道挑战的保护相关。这个 这个项目的总体目标是扩大我们对 SIV减毒活疫苗产生的细胞免疫应答 菌株，包括系统和粘膜部位，并进行 更明确地解决细胞介导的作用的实验 保护性免疫中的免疫反应。细胞免疫 感染SIV减毒疫苗引起的反应将是 进一步鉴定，包括分析抗原特异性细胞因子 抑制SIV的可溶性因子的反应和特性 复制。细胞毒性T淋巴细胞反应将与 保护免受SIV异源毒株的攻击。这个 我们将先分析粘膜部位的细胞免疫反应。 按照黏膜免疫路线进行免疫。最后，领养 T细胞在基因完全相同的恒河猴中的转移将是 用来确定细胞介导的免疫反应在 调解保护性免疫。来自这些研究的信息应该 提供有价值的信息，说明细胞介导的免疫 反应在介导对SIV和SIV挑战的抵抗中发挥作用 爱滋病毒。资助美国国立卫生研究院AI-43044(项目2，分包)出版物 Zelinski-Wooten MB，Hutchison JS，Hess DL，Wolf DP，Stouffer，RL.一个 周期中期推注重组人卵泡刺激素 多个卵泡发育后诱发排卵周围事件 在猕猴身上。哼唱，1998年，13：554-560。史密斯·杜德、萨度·A、狼 Dp.恒河猴卵母细胞短暂暴露于Calyculin-A和 冈田酸刺激生发泡破裂 随后的发育和受精。生物报告58：880-886 1998年。刘DS，康纳·韦，Wolf DP，Alexander M.分布不均匀 恒河猴头部和尾巴中的桥索甾醇和二十二碳六烯酸 猴子的精子。J Lipid Res 39：1404-1411,1998。迈尔霍费尔·A，史密斯 首页--期刊主要分类--期刊细介绍--期刊题录与文摘--期刊详细文摘内容卵母细胞是 灵长类动物卵巢中儿茶酚胺的来源--一篇小说的证据 细胞-细胞调控环。《美国学报》95：10990-10995， 1998年。首页--期刊主要分类--期刊细介绍--期刊题录与文摘--文摘内容 人粘粒探针在恒河猴体内的定位 荧光原位杂交。遗传技术杂志24：37-43， 1998年。Wolf DP，孟L，Ely JJ，Stouffer RL.的最新进展 哺乳动物克隆。J Reprod Genet 15：234-238,1998。史密斯·格德 萨杜A，数学S，沃尔夫民主党。蛋白质磷酸酶的特性研究 在小鼠卵母细胞中，Dev Biol 204：537-549,1998。米克森·巴，巴顿·B Wolf DP，Larson J.宫内受精后妊娠率 (UI)使用冷冻后处理的供体精子与冷冻前处理的供体精子。 肥育(增刊)，p-605,1998(摘要#S-317)。拉尔森·J·M Mixon BA，Albin IA，Tep NL，Wolf DP，Johnson A.内部精液 分析能力测试以确保技术人员之间和内部 统计控制。肥精(Suppl)，p-607,1998(摘要 #S-317.首页--期刊主要分类--期刊细介绍--期刊题录与文摘--期刊详细文摘内容 沃尔夫民主党。采用胚泡培养计划。不孕不育 《方案补编0-266》，1998(摘要599)。

Despite intensive study, the immune responses associated with protective immunity against HIV infection remain poorly understood. Analysis of cell-mediated immune responses induced by live attenuated SIV offers an ideal research setting in which to define specific mechanisms that may play a role in mediating protection. Rhesus macaques vaccinated with SIV239?nef or SIV239?3 develop vigorous SIV-specific cytotoxic T lymphocyte response and CD8+ lymphocytes from animals infected with live-attenuated SIV strains are able to inhibit SIV replication through both MHC-restricted mechanisms and the production of soluble factors. Prospective analysis of SIV-specific CTL responses in animals infected with different live attenuated SIV strains suggests that induction of strong SIV-specific immune responses correlates with protection against vaginal challenge. The overall goal of this project is to extend our understanding of cell-mediated immune responses generated by live attenua ted SIV strains, both at systemic and mucosal sites, and to carry out experiments that more definitively address the role of cell-mediated immune responses in protective immunity. The cell-mediated immune responses induced by infection with attenuated SIV vaccines will be further characterized, including analysis of antigen-specific cytokine responses and characterization of soluble factors able to suppress SIV replication. Cytotoxic T lymphocyte responses will be correlated with protection against challenge with heterologous strains of SIV. The cell-mediated immune responses in mucosal sites will be analyzed prior to, and following, mucosal routes of immunization. Finally, adoptive transfer of T cells in genetically identical rhesus macaques will be utilized to determine the role of cell-mediated immune responses in mediating protective immunity. Information from these studies should provide valuable information about the role that cell-mediated immune responses play in mediated resistance against challenge with SIV and HIV. FUNDING NIH AI-43044 (Project 2, subcontract) PUBLICATIONS Zelinski-Wooten MB, Hutchison JS, Hess DL, Wolf DP, Stouffer, RL. A bolus of recombinant human follicle stimulating hormone at midcycle induces periovulatory events following multiple follicular development in macaques. Hum Reprod 13:554-560, 1998. Smith GD, Sadhu A, Wolf DP. Transient exposure of rhesus macaque oocytes to calyculin-A and okadaic acid stimulates germinal vesicle breakdown permitting subsequent development and fertilization. Biol Reprod 58:880-886, 1998. Liu DS, Connor WE, Wolf DP, Alexander M. Uneven distribution of desmosterol and docasahexaenoic acid in the heads and tails of rhesus monkey sperm. J Lipid Res 39:1404-1411, 1998. Mayerhofer A, Smith GD, Danilchik M, Levine J, Wolf DP, Dissen GA, Ojeda SR. Oocytes are a source of catecholamines in the primate ovary Evidence for a novel cell-cell regulatory loop. Proc Natl Acad Sci USA 95:10990-10995, 1998. Lawce M, Olson S, Wolf DP, Magenis R, Ellen R. Comparative mapping of human cosmid probes in rhesus monkey (Macaca mulatta) using fluorescence in situ hybridization. J Assoc Genetic Tech 24:37-43, 1998. Wolf DP, Meng L, Ely JJ, Stouffer RL. Recent progress in mammalian cloning. J Assist Reprod Genet 15:234-238, 1998. Smith GD, Sadhu A, Mathies S, Wolf DP. Characterization of protein phosphatases in mouse oocytes, Dev Biol 204:537-549, 1998. Mixon BA, Patton B, Wolf DP, Larson J. Pregnancy rates following intrauterine insemination (UI) with post-freeze processed vs. pre-freeze processed donor sperm. Fertil Steril (Suppl), p-605, 1998 (abstract #S-317). Larson JM, Mixon BA, Albin IA, Tep NL, Wolf DP, Johnson A. Internal semen analysis proficiency testing to ensure inter- and intra-technician statistical control. Fertil Steril (Suppl), p-607, 1998 (abstract #S-317). Sadler-Fredd K, Patton PE, Larson J, Eaton DL, Teramura D, Wolf DP. Adoption of a blastocyst culture program. Fertil Steril Program Supplement 0-266, 1998 (abstract 599).