CORE--HYBRIDOMA

杂交瘤核心实验室为 单抗生产的方方面面的计划，包括 1)动物(Balb/c小鼠)免疫，2)动物血清筛选 免疫反应，3)执行融合方案和所有细胞培养 关于稳定杂交瘤细胞的产生、克隆和建立 细胞系，4)使用ELISA法进行单抗的筛选程序， 免疫印迹、免疫荧光显微镜和流式细胞术，5) 从腹水中制备和纯化精选单抗，6)同种分型 单抗，以及7)结合选定的单抗用于特定实验(即至 FITC或生物素)。

The Hybridoma Core Laboratory provides a centralized resource for the Program for all aspects of monoclonal antibody (mAb) production including 1) immunization of animals (Balb/c mice), 2) screening of animal sera for immune responses, 3) performing fusion protocols and all cell culture related to the production, cloning and establishment of stable hybridoma cell lines, 4) performing screening protocols for mAbs using ELISA, Western blot, immunofluorescence microscopy, and flow cytometry, 5) producing and purifying selected mAbs from ascitic fluid, 6) isotyping mAbs, and 7) conjugating selected mAbs for particular experiments (ie. to FITC or biotin).