CENTRAL NERVOUS SYSTEM MEDIATION OF AFFECTIVE AUTONOMIC/BEHAVIORAL RESPONSES

这些实验的总体目标是增进我们对 这些反应的神经生理机制 在动物模型中，交感神经系统对情绪应激源的反应。 项目4侧重于两个集成的自主/行为反应，以 交感神经系统占主导地位的情绪应激源； 防御反应和警觉反应：这个项目寻求 描绘调节心血管系统的功能传出通路 防御和警觉反应的组成部分，并确定如何 关键的中枢神经系统站点相互作用，以产生这些集成模式 对压力源的反应。虽然心血管反应性的减弱 是许多临床干预的目标，中枢神经系统的机制参与其中 情绪反应系统的增益调节较差 明白了。因此，当前提案的目标之一是 评估来自化学感受器的感觉信息的机制 和压力感受器调节与 防御反应和警觉反应。通过使用 电生理、神经解剖学和行为学技术 将解决以下具体目标：1)继续 在上一个资助期内发起的查询将使用 逆行分析追踪介导动脉粥样硬化的传出通路 防御反应和警觉反应。2)使用利多卡因 可逆剥离技术和单电池记录技术 确定位于杏仁核的整合部位的功能， 下丘脑和中脑导水管周围灰质(PAG) 调节防御反应；3)使用利多卡因皮损和单用 细胞记录技术用于评价细胞反馈调节机制 根据压力感受器和压力感受器信息的心血管反应性 化学感受性传入；4)使用逆行分析绘制通路图 从压力感受器到中枢神经系统的反馈调节 防御反应。

The overall objective of these experiments is to advance our understanding of the neurophysiological mechanisms that underlie responses of the sympathetic nervous system to emotional stressors in an animal model. Project 4 focuses upon two integrated autonomic/behavioral responses to emotional stressors in which the sympathetic nervous system predominates; the defense reaction and the vigilance reaction: This project seeks to delineate the functional efferent pathways that mediate the cardiovascular components of the defense and vigilance reactions and to determine how critical CNS sites interact to produce these integrated patterns of response to stressors. Although attenuation of cardiovascular reactivity is a goal of many clinical interventions, the CNS mechanisms involved in the modulation of the gain of affective response systems are poorly understood. Accordingly, one of the objectives of the current proposal is to assess the mechanisms by which sensory information from chemoreceptor and baroreceptors modulate cardiovascular reactivity associated with the defense reaction and the vigilance reaction. By using electrophysiological, neuroanatomical, and behavioral techniques the following specific aims will be addressed; 1) to continue the line of inquiry initiated during the previous funding period which will use a retrograde analysis to trace the efferent pathways that mediate athe defense reaction and the vigilance reaction. 2) to use the lidocaine reversible lesioning technique and single cell recording techniques to determine the function of integrative sites located ina the amygdala, hypothalamus and periaqueductal grey (PAG) ina the organization and regulation the defense reaction; 3) to use lidocaine lesions and single cells recording techniques to asses mechanisms of feedback regulation of cardiovascular reactivity by information from baroreceptor and chemoreceptive afferents; 4) to use a retrograde analysis to map pathways from baroreceptors to CNS sites that mediate feedback regulation of the defense reaction.