DEVELOPMENT OF FIELD FREQUENCY LOCKS FOR L BAND SPECTROMETERS

尽管有丰富的硝酸化学知识 氧化物（NO），关于反应存在相当大的不确定性 体内NO 这项研究的一个目标是描述 一氧化氮与蛋白质结合铁的相互作用，通常与 硫醇配体。 研究的第二个目标是使用 二硫代氨基甲酸酯作为小鼠体内NO的自旋陷阱， 通过L-波段EPR光谱实时检测NO， 通过X波段光谱学对单个器官进行体内分析。 这 本技术已成功地用于检测小鼠NO产生 脓毒性休克模型，利用二乙基二硫代氨基甲酸酯（DETC）和 沿着注入N-甲基-D-葡糖胺二硫代氨基甲酸盐和铁（II） 硫酸盐和柠檬酸钠。 观察到的EPR信号是典型的 一种单亚硝酰铁络合物;在L波段光谱学中，它由以下组成： 基本对称的三重态，分裂约为 12.8因为氮气的缘故。 在目前的试验中， 由Fe-DETC-NO复合物产生的具有更大的强度，可能是由于 其更大的亲脂性和在细胞中浓缩的能力 膜。 未来的工作将涉及测试各种其他 二硫代氨基甲酸盐，包括肌氨酸、吡咯烷和脯氨酸 衍生物，以最大限度地从捕获NO的光谱中， 肝、肾、脑、脊髓和循环中。

Despite the wealth of knowledge about the chemistry of nitric oxide (NO), there is considerable uncertainty regarding the reactions of NO in vivo. One goal of this research is to characterize the interaction of nitric oxide with protein-bound iron, typically with thiol ligands. A second goal of the research is to use dithiocarbamates as in vivo spin traps for NO in mice, allowing the real-time detection of NO through L-band EPR spectroscopy and the ex vivo analysis of individual organs through X-band spectroscopy. This technique has been successful for detecting NO production in mouse septic shock models, utilizing diethyl dithiocarbamate (DETC) and N-methyl-D-glucamine dithiocarbamate injected along with iron(II) sulfate and sodium citrate. The EPR signal observed is characteristic of a mononitrosyl iron complex; in L-band spectroscopy it consists of an essentially symmetrical triplet with splitting of approximately 12.8 Gauss due to the nitrogen. In the current trials, the signal produced by the Fe-DETC-NO complex has a greater intensity, likely due to its greater lipophilicity and ability to concentrate in cell membranes. Future work will involve testing a variety of other dithiocarbamates, including the sarcosine, pyrrolidine, and proline derivatives, in order to maximize the spectrum from trapped NO in the liver, kidney, brain, spinal cord, and in circulation.