POLYMORPHIC UPSTREAM STRUCTURE & PFG 27/25 GENE CONTROL

描述（改编自申请人的摘要）：疟原虫 恶性疟原虫极早期配子体期基因Pfg 27/25具有多态性， 基因座上游的编码序列，其周围是保守的 序列的 多态性区域的结构指定准确或 改变了基因的血液阶段特异性转录。 多态 这种结构是由一种至今还不为人知的DNA重排引起的。 的 本提案的总体目标是确定详细结构 在实验室分离株中， Pfg 17/25基因的特异性转录模式，以及 这些多态性在自然界中存在。 Pfg 27/25的5'末端 mRNA也将被确定，以查看转录起始位点 相对于多态域的边界发生。

DESCRIPTION (Adapted from the Applicant's Abstract): The Plasmodium falciparum very early gametocyte stage gene, Pfg27/25, has a polymorphic locus upstreasm of the coding sequences which is surrounded by conserved sequences. The structure of the polymorphic region specifies accurate or altered blood stage specific transcription of the gene. The polymorphic structures arise by an as yet uncharacterizes DNA rearrangement. The overall objectives of this proposal are to determine the detailed structure of this locus in laboratory isolates that display different blood satge specific transcription patterns for the Pfg17/25 gene, and the extent to which these polymorphisms occur in nature. The 5' terminus of the Pfg27/25 mRNAs will also be determined to see where the transcription start sites occur with respect to the boundaries of the polymorphic domain.