ZEISS LSM 510 CONFOCAL MICROSCOPE

在过去的三年里，范德比尔特大学的数字成像技术发展迅速。其中一些是由于新教师的加入，但更多的是由于细胞成像共享资源在教授研究人员使用共聚焦显微镜方面的成功，这是范德比尔特学院目前可用的一种仪器无法满足的。为了弥补这一缺陷，现有共聚焦显微镜的几个知名用户开始讨论分摊新仪器的使用和维护费用的计划。本提案中包含的主要用户妥协了一个核心组，共享相关的研究目标并使用类似的技术。例如，Denison和Solnica-Krezel都在对活细胞和胚胎进行延时成像实验，至少还有两名研究者（Hogan和Wright）希望使用这种方法。PI Miller与细胞成像资源主任Dave Piston合作，开发了LSM510多色gfp成像方法。Denison， Solnica-Kerezel和Miller打算在他们的项目中利用GFP多路复用。一些主要用户将使用免疫化学或细胞学染料对固定组织进行多色标记（Denison, Hogan, Wright, Solnica-Kerezel）。计算机控制的LSM510（458,488,514,633）的多条激光线提供了这些研究人员所需的全部激发波长和功率。虽然显微镜将主要由主要用户使用，但显微镜时间将通过细胞成像共享资源分配，以确保分配是公平的，并且未安排的时间可以由范德比尔特的其他研究人员使用。

There has been a rapid growth in the use of digital imaging at Vanderbilt over the last three years. Some of this due to newly faculty, but much of it has arisen from success of the Cell Imaging Shared Resource in teaching researchers to use the confocal microscopy cannot be met by the one instrument that is currently available to Vanderbilt faculty. In an effort to remedy this deficit, several well established users of the existing confocal microscope began discussion a plan for sharing the use and maintenance costs of a new instrument. The Major Users included in this proposal compromise a core group share related research goals and employ similar techniques. For example, both Denison and Solnica-Krezel are performing time-lapse imaging experiments on live cells and embryos and at least two other investigators (Hogan, Wright) anticipate using this approach. The PI, Miller, in collaboration with Dave Piston, the Director of the Cell Imaging Resource, have developed methods for multicolor-GFP imaging in the LSM510. Denison, Solnica-Kerezel and Miller intend to exploit GFP multiplexing in their projects. Severak of the Major Users will employ multicolor labeling in fixed tissues with immunochemical or cytological dyes (Denison, Hogan, Wright, Solnica-Kerezel). The computer controlled, multiple laser lines of the LSM510 (458, 488, 514, 633) provide the full range of excitation wavelengths and powers needed by these investigators. Although the microscope will be largely utilized by the Major Users, microscope time will be allocated through the Cell Imaging Shared Resource to insure that allotments are fair and that unscheduled time can be used by other investigators at Vanderbilt.