MULTIPHOTON SCANNING LASER FLUORESCENCE MICROSCOPE

该共享仪器拨款申请申请资金用于购买一台蔡司LSM 510 NLO多光子激光扫描显微镜，安装在俄勒冈大学生物光学实验室。该显微镜将用于推进正在进行的NIH支持的研究，在俄勒冈大学神经科学和分子生物学研究所的许多实验室中，包括六个主要用户，他们描述了具体的项目，这些项目将通过多光子荧光显微镜的可用性大大促进。这项新技术提供的两个主要好处是：(1)在活体标本上进行延时显微镜的能力，大大减少了光动力损伤或荧光标记的漂白；(2)能够在标本100 μ m或更深的深度获得清晰的图像。其中五个主要用户研究斑马鱼（Judith Eisen， Charles Kimmel和John Postlethwait）、果蝇（Chris Doe）、被囊动物（John Postlethwait）和秀丽隐杆线虫（Bruce bowman）的发育生物学。一个用户（威廉·罗伯茨）研究突触生理学和钙信号在青蛙耳朵的感觉受体。这台显微镜将作为共享设备提供给俄勒冈大学的生命科学家使用。

This Shared Instrumentation Grant proposal requests funds to purchase a Zeiss LSM 510 NLO multiphoton laser scanning microscope, to be installed in the Bio-Optics Laboratory at the University of Oregon. This microscope will be used to advance ongoing NIH supported research in numerous laboratories in the Institutes of Neuroscience and Molecular Biology at the University of Oregon, including six major users who have described specific projects that will be greatly facilitated by the availability of a multi-photon fluorescence microscope. The two main benefits offered by this new technology are (1) the capability performing time-lapse microscopy on living specimens with greatly reduced photodynamic damage or bleaching of fluorescent labels and (2) the ability to obtain crisp images at depths of 100 mu m or more into a specimen. Five of the major users study the developmental biology of zebrafish (Judith Eisen, Charles Kimmel and John Postlethwait), Drosophila (Chris Doe), tunicates (John Postlethwait) and C. elegans (Bruce Bowerman). One user (William Roberts) studies synaptic physiology and calcium signaling in sensory receptors in the frog ear. The microscope will be available as a shared facility for use by life scientists at the University of Oregon.