PARAMYXOVIRUS MEMBRANE FUSION

该项目的长期目标是确定病毒糖蛋白 直接膜融合膜融合是生命的基本步骤 循环的包膜病毒，并负责渗透的 宿主细胞某些类型的病毒，特别是副粘病毒，如 纽卡斯尔病病毒（NDV）在中性pH下可与血浆融合 宿主细胞的膜。这些病毒还具有 诱导合胞体形成，这是一种重要的细胞病变效应， 病毒通过组织传播的另一种方式。 NDV膜融合需要在相同的膜中存在NDV融合蛋白。 两种病毒糖蛋白，HN蛋白和F蛋白。电流 有证据表明，HN蛋白的作用是激活 直接负责融合的F蛋白的活性 活动此外，目前的证据表明， 合胞体的形成，以及测试 该模型构成了本提案的基础。该项目将利用 HN和F蛋白基因突变体的广泛收集。 具体目标： 1.为了确定融合蛋白如何指导攻击之间的融合， 和靶细胞膜。 2.为了验证HN蛋白的功能是激活 融合蛋白，并确定参与激活的机制。

The long-term goal of this project is to determine how viral glycoproteins direct membrane fusion. Membrane fusion is a fundamental step in the life cycle of enveloped viruses and is responsible for the penetration of the host cell. Some types of viruses, notably paramyxoviruses such as Newcastle disease virus (NDV), can fuse at neutral pH with the plasma membrane of the host cell. These viruses also have the property of inducing syncytia formation, an important cytopathic effect as well as an alternate means of virus spread through a tissue. NDV membrane fusion requires the presence, in the same membrane, of the two viral glycoproteins, the HN protein and the F protein. Current evidence suggests the hypothesis that the HN protein serves to activate the activity of the F protein which is directly responsible for the fusion event. Furthermore, current evidence suggests a sequence of events leading to the formation of syncytia, and experiments to test the predictions of this model form the basis of this proposal. The project will make use of an extensive collection of mutants in both the HN and F protein genes. Specific Aims: 1. To define how the fusion protein directs the fusion between the attack and the target membranes. 2. To test the hypothesis that the HN protein functions to activate the fusion protein and to define the mechanisms involved in activation.