IN UTERO PROTEINASE ACTIVITY DURING HAMSTER EMBRYO ZONA ESCAPE TIME WINDOW

目前，体内肿瘤逃逸的机制尚不清楚， 正如有助于体外"孵化"的因素一样， 从透明珊瑚中分离出来 本研究的总体目标是 了解体内肿瘤的影响因素 通过研究冲洗液中酶活性的存在， 与逃逸时间窗口（冈萨雷斯和 Bavister，1995）。 妊娠期间从子宫采集胚胎和冲洗液 逃逸时间窗口将用于比较 卵子/胚胎，它们在生殖道中的位置，以及 在妊娠、假孕和非妊娠中存在酶活性 仓鼠的怀孕子宫。 因此，这一目标 建议的研究是：1）确定是否有子宫酶 可能导致体内溶血的活性， 与妊娠期和妊娠期的子宫内膜异位症逃逸时间窗一致， 假孕仓鼠，2）为了检验假设， 在非妊娠的自行车仓鼠雌性中的溶血活性和3）至 确定体外溶血活性是否不同于 体内裂解活性。 在这项研究中，每个人的贡献 参与脱卵的参与者（透明卵，胚胎 和子宫上皮）进行检查，以获得更完整的 了解子宫内流产的机制。 本研究 也将为进一步的研究提供基础，以阐明1） 子宫酶活性的调节作用与 关键的着床前时期，和2）可能的胚胎-母体 对话

At present, the mechanism of zona escape in vivo is poorly understood, as are the factors contributing to in vitro "hatching" from the zona pellucida. The overall goal of the present study is to obtain an understanding of the contributing factors of in vivo zona escape by studying the presence of enzyme activity in flushates that are coincident with the zona escape time window (Gonzales and Bavister, 1995) in both pregnant and pseudopregnant hamsters. Collections of embryos and flushates from the uterus during the zona escape time window will be used to compare the morphology of eggs/embryos, their location in the reproductive tract, and the presence of enzymatic activity in pregnant, pseudopregnant, and non- pregnant uteri of hamsters. Therefore, the objectives of this proposed study are: 1) to determine if there is a uterine enzyme activity that is potentially responsible for zona lysis in vivo that is coincident with the zona escape time window in both pregnant and pseudopregnant hamsters, 2) to test the hypothesis that there is no zona lytic activity in non-pregnant cycling hamster females and 3) to determine if zona lytic activity is different in vitro than the zona lytic activity in vivo. In this study the contribution of each participants involved in zona escape (the zona pellucida, the embryo and the uterine epithelium) is examined to achieve a more complete understanding of the mechanism of zona loss in utero. This study will also provide a foundation for further research to elucidate 1) regulatory effect of uterine enzyme activity associated with the critical preimplantation period, and 2) possible embryo-maternal dialogue.