HUMAN PERIPHERAL BLOOD STUDIES OF MUTATIONS IN THE ABERJONA REGION

描述：泥炭沼泽沉积物岩心的化学分析 近市政G井和H井在上部的类似分析证实 神秘湖表明，大量重金属包括 铬和砷在早期过后的某个时间经过井位 S。水文地质模型表明，大约60%的水来自于 G井和H井在1964年至1979年夏季打来的水 来自阿伯乔纳河。根据沉积记录估算流量 并因地制宜，计算出铬 砷浓度分别为200ug/L和50ug/L 存在于东沃本的饮用水中。这相当于 对人体细胞有明显致突变性的4muM铬酸盐 在5个小时的暴露时间内。这种治疗的突变谱有 在体外检测到人HPRT基因外显子3。 现在有人提议研究血细胞样本中的突变谱。 来自东沃本、西沃本、阿灵顿和西梅德福德的居民 发现铬的遗传效应是否可以在暴露的环境中发现 居民。 在此之前，有人建议收集儿童头发的样本 用于分析，以发现是否以及何时发生了接触。vt.给出 确认结果，他们将利用他们的社区外展努力 解释他们的技术的目标、方法和局限性。他们 建议招收五个多代家庭，他们集体 代表男女、年龄分布广泛和 欧洲和非洲血统。尤其是有两个家庭 主要是在东沃本1960-1990年将寻找谁的来源 饮用水是市政供水。 在进行公众教育和征集活动的同时，他们 建议实现所需的某些额外技术目标 在人类血液样本中进行简便和准确的突变光谱分析。 这些包括改进的样品制备方法，探索 某些多拷贝核DNA序列的适合性，开发 内部标准和实验室规程的制定，以确保 质量、准确性和捐赠者的保密性。 最初，他们计划从50个突变谱中获得约50个突变谱。 捐赠者。他们希望能够辨别出基因突变谱是否 持续饮酒的人的DNA样本中含有铬酸盐 来自G井和H井的水，在未接触过的人中不存在。他们 然后希望看看家族内部的光谱是否存在差异 基于年龄或性别；然后他们希望能够发现 在我们的家庭内部或家庭之间存在着显著的差异 学习。 如果成功，他们将为一种新的有价值的方式奠定基础 发现人类种群中突变的数量和种类，以及 提供识别环境因素的方法，如果有的话， 人类的变化。

Description: The chemical analysis of sediment cores in the peat marsh near municipal wells G and H confirmed by similar analysis in the Upper Mystic Lake indicates that a massive bolus of heavy metals including chromium and arsenic passed by the well sites sometime after the early 1960's. Hydrogeologic models show that some 60% of the water drawn from wells G and H water drawn in the summers of 1964 to 1979 had to come from the Aberjona River. Based on the sedimentary record estimated flow rates and modelling of local conditions, it is calculated that chromium and arsenic concentrations of 200 mug/L and 50 mug/L respectively were present in the drinking water of East Woburn. This is equivalent to 4muM chromate which was found to be markedly mutagenic to human cells in a 5 hour exposure. The mutational spectrum of this treatment has been determined in exon 3 of the human hprt gene in vitro. It is now proposed to study the mutational spectra in blood cells sampled from residents of East Woburn, West Woburn, Arlington and West Medford to discover if the genetic effects of chromium may be found in exposed residents. Before this, it is proposed that samples of childhood hair be collected for analysis to discover if and when exposure occurred. Given confirmatory results, they will use their community outreach effort to explain the goals, methods and limitations of their technology. They propose to enroll five multi-generational families who collectively represent persons of both genders, a wide distribution of ages and European and African ancestry. In particular two families living primarily in East Woburn 1960-1990 will be sought whose source of drinking water was the municipal water supply. In parallel with this public education and solicitation effort they propose to pursue certain additional technical goals which are needed for facile and accurate mutational spectrometry in human blood samples. These involve improved methods for sample preparation, exploration of certain multicopy nuclear DNA sequences for suitability, development of internal standards and development of laboratory protocols to assure quality, accuracy and donor confidentiality. Initially they propose to obtain some fifty mutational spectra from fifty donors. They hope to be able to discern if the mutational spectrum of chromate is present in the DNA samples of persons who consistently drank water from wells G and H and not present in unexposed individuals. They then hope to see if there are differences among spectra within families on the basis of age or gender; they then hope to be able to discover if there are significant differences within or among families in our studies. If successful, they will have laid the basis for a new and valuable way to discover the amount and kinds of mutations in human populations and offer the means to identify environmental causes, if any, of genetic change in humans.