GONADOTROPIN STRUCTURE

我们正在努力阐明这三个 蛋白质人绒毛膜促性腺激素的空间结构 X射线晶体学和NMR光谱学。 对于后一种技术， 促性腺激素的生产具有高水平的替代性， 13 C和15 N。 质谱法提供了一种精确的方法， 评估这些同位素掺入到 蛋白 这些决定是在国家一级作出的。 单个氨基酸、选定的肽以及完整的 蛋白 描述后一项工作的论文已发表在 J. Biomolecular NMR 7（1996）295-304.

We are undertaking a major effort to elucidate the three dimensional structure of the protein human chorionic gonadotropin via x-ray crystallography and NMR spectroscopy. For the latter technique, gonadotropin is being produced with a high level of substitution of 13C and 15N. Mass spectrometry provides an accurate means of assessing the levels of incorporation of these isotopes into the protein. The determinations are being made at the level of the individual amino acids, selected peptides, as well as the intact protein. A paper describing the latter work has been published in the J. Biomolecular NMR 7 (1996) 295-304.