INFRARED WAVE LENGTH MATRIX-ASSISTED LASER DESORPTION MASS SPECTROMETRY

使用UV波长激光照射的MALDI-MS已经成为广泛的 采用的技术用于需要MW的生物应用 肽和蛋白质的测定。 红外波长激光器 辐射很少用于MALDI-MS应用。 一 IR MALDI研究量小的原因是缺乏 一个方便的商业红外波长激光器， 持续时间短。 迄今为止，所有研究均使用Er（YAG）激光器进行 具有2.94 μ m的固定波长和在该范围内的脉冲持续时间 100 - 200 ns。 最近的商业可用性的光学 参量振荡器（OPO），允许随时调谐激光器 波长范围为0.7 - 4.0 μ m， 持续时间为~ 6 ns的辐照使我们计划 对IR MALDI进行彻底的重新调查 IR的研究 MALDI正在进行使用Surelite 11 Nd（YAG）泵浦激光器， 与Mirage 3000 OPO（Continuum，Santa Clara，CA）组合。我们 成功地将OPO的输出耦合到我们的第二个客户端 建立了MALDI-TOF仪器，并进行了广泛的计划 新仪器的研究。 我们已经做了一些 在出版的论文中报道了有趣的发现（Niu等人， J. Am. Soc. Mass Spectrom.）。这些发现具有潜在价值 从实际和基本的角度来看（为了获得 了解MALD-MS过程）。

MALDI-MS using UV wavelength laser irradiation has become a widely adopted technique for biological applications that require MW determination of peptides and proteins. IR wavelength laser irradiation has been much less used for MALDI-MS applications. One reason for the small amount of research in IR MALDI has been the lack of a convenient commercial IR-wavelength laser that produces pulses of short duration. All studies to date carried out using Er(YAG) lasers with a fixed wavelength of 2.94 prn and pulse duration in the range 100 - 200 ns. The recent commercial availability of optical parametric oscillators (OPO) that allow ready tuning of the laser wavelength in the range 0.7 - 4.0 prn and intense pulses of irradiation with duration - 6 ns has made it attractive for us to plan a thorough reinvestigation of IR MALDI. This investigation of IR MALDI is being undertaken using a Surelite 11 Nd(YAG) pump laser in combination with a Mirage 3000 OPO (Continuum, Santa Clara, CA.). We have successfully coupled the output of the OPO to our second custom built MALDI-TOF instrument and have carried out an extensive program of research with the new instrument. We have made a number of interesting findings that are reported in a paper in press (Niu et al, J. Am. Soc. Mass Spectrom.). These findings are of potential value both from a practical and a fundamental point of view (for obtaining an understanding of the MALD-MS process).