CORE--GENE ANALYSIS

这三个研究项目都有一个共同的目标 在揭示Ets家族的发育和致癌功能方面 转录因子。虽然不同的系统中使用的 在个别项目中，许多实验性方法是所有项目共同的。一 这项拨款提案的基本理念是，该中心 分子和结构生物学（CMSB）可以提供共享设施， 拟议研究项目的技术专业知识;以及 整合资源，我们将能够负担得起的有效和 为实现这一目标提供经济支持。 基因表达模式的检查和可能的 下游基因是理解ets基因功能的核心。在 根据这一理解，两个领域的实验需要， 被确定为必不可少的，可以通过核心 设施它们是（A）DNA序列分析和（B）原位检测 基因表达模式。 该核心的目标是：1）为以下方面提供统一的资源 DNA序列分析2)提供标准化方法和共享 用于检测基因表达模式的设备。

All three research projects in this grant proposal share the common goal in unraveling the developmental and oncogenic functions of the Ets family of transcription factors. Although different systems are used in the individual projects, many experimental approaches are common to all. One underlying philosophy of this grant proposal is that the Center for Molecular and Structural Biology (CMSB) can provide share facility and technical expertise to the proposed research projects; and by consolidating the resources we will be able to afford efficient and economical support for the objectives of this grant proposal. Examination of gene expression patterns and characterization of possible down-stream genes are central to understanding the ets gene functions. In accordance with this understanding, two areas of experimental needs have been identified to be essential and can be best satisfied by core facilities. These are (A) DNA sequence analysis and (B) in situ detection of gene expression patterns. The objective of this core are: 1) To provide consolidated resource for DNA sequence analysis. 2) To provide standardized methodology and shared facilities for examining gene expression patterns.