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CORE--TISSUE CULTURE, VIRUS AND ANTIBODY FACILITY

CORE--TISSUE CULTURE, VIRUS AND ANTIBODY FACILITY
核心——组织培养、病毒和抗体设施
批准号：
6300576
负责人：
STEPHEN Paine GOFF
金额：
$11.73万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
项目状态：
已结题
起止时间：
2000-01-31 至 2000-12-31
来源：
https://reporter.nih.gov/project-details/6300576
关键词：
Abelson leukemia virus biomedical facility bone marrow cell transformation cryopreservation fibroblasts laboratory mouse microorganism resource /registry molecular cloning monoclonal antibody tissue /cell culture tissue resource /registry transfection

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中文摘要
翻译
这个核心将为所有四个人提供组织培养衍生试剂 项目核心将制备和滴定突变体和野生型的 V-ABL和BCR-ABL病毒。核心也会利用这些病毒 体外转化小鼠骨髓细胞和成纤维细胞系， 在体内感染小鼠。核心还将从不同的 项目野生型和突变形式的Jak和Abi-1蛋白，并将产生 稳定转染的细胞系以可诱导的方式表达这些蛋白， 方式核心将产生单克隆抗体， 所有项目的常规细胞培养和冷冻
英文摘要
This core will provide tissue culture-derived reagents for all four projects. The core will make and titer stocks of mutant and wt forms of v-abl and bcr-abl viruses. The core will also use these viruses to transform murine bone marrow cells and fibroblast ell lines in vitro and to infect mice in vivo. The core will also obtain from different projects wt and mutant forms of Jak and Abi-1 proteins and will generate stably transfected lines expressing these proteins in an inducible manner. The core will generate monoclonal antibodies and will provide routine cell culture and freezing for all of the projects.
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