MICROSATELLITES AS MARKERS FOR BLADDER CANCER

高通量微卫星分析识别肿瘤特异性基因 与膀胱癌进展相关的改变。拟议中的工作 寻求探讨与膀胱癌治疗相关的几个临床问题 基于对原发肿瘤中这些基因变化的检测 尿液。这项计划的具体目标是：(1)界定 微卫星标记板检测的灵敏度和特异度 检测复发的浅表性膀胱癌和，(2) 初步探讨微卫星的特定模式是否 基因改变(即杂合性缺失或不稳定)与罹患 浅表性和侵袭性疾病的复发或进展 癌症。这些具体目标将通过前瞻性的方式实现 日本血吸虫病患者肿瘤和尿液标本的微卫星分析 浅表性和浸润性膀胱癌的治疗 在约翰·霍普金斯医院接受治疗。分析将是 使用项目1中开发的分子方法和技术进行 2.这个项目的更大目标是奠定一个实际的基础 用于监测的微卫星分析的未来研究和 为不同阶段的患者建立风险档案 膀胱癌。

High throughput microsatellite analysis identifies tumor-specific genetic alterations associated with bladder cancer progression. The proposed work seeks to explore several clinical issues related to bladder cancer managed based on the detection of these genetic changes in primary tumors and urine. The specific aims of this project are: (1) to define the sensitivity and specificity of microsatellite marker panels for the detection of recurrent superficial bladder cancers and, (2) to preliminarily explore whether specific patterns for microsatellite alterations (i.e., LOH or instability) correlate with increased risk of disease recurrence or progression in both superficial and invasive cancers. These specific aims will be accomplished through prospective microsatellite analysis of paired tumor and urine specimens obtained from patients with superficial and invasive bladder cancers undergoing therapy with curative intent at the John Hopkins Hospital. The analyses will be performed using molecular methods and technology developed in projects #1 and 2. The larger goal of this project is to lay a practical foundation for future studies of microsatellite analysis for monitoring and for the establishment of risk profiles for patients with different stages of bladder cancer.