TWO PHOTON FLUORESCENCE CORRELATION SPECT OF RHODAMINE GREEN IN SOLUTION

在单光子和多光子荧光显微镜中，光漂白 在强光下的荧光标记是一个限制因素。 荧光团在强光照射下会发生光漂白。 通过单分子或双分子反应，其中 光子能量和环境起着主要的作用。可靠 测量光漂白量子产额(？B)的技术有 对从根本上理解这些机制和途径至关重要 参与漂白动力学的研究。在单分子水平上， 荧光相关光谱(FCS)是一种功能强大的技术 用于扩散以及三重态的表征。理论上的 已进行分析，以扩大功能界别在以下方面的使用 光漂白研究。在这些分析中，三胞胎-首先是兴奋 单线态被认为是可能的光漂白途径。 环境效应，如单线态氧和外部重原子 影响，也进行了讨论。

In one- and multi-photon fluorescence microscopy, photobleaching of the fluorescent markers under intense light is a limiting factor. A fluorophore under an intense illumination can undergo photobleaching through either unimolecular or bimolecular reaction in which the photon energy and the enviroment play a major rule. Reliable techniques for photobleaching quantum yield (?b) measurements are crucial for fundamental understanding of the mechanisms and pathways involved in bleaching dynamics. On a single molecule level, fluorescence correlation spectroscopy (FCS) is a powerfull technique for diffusion as well as triplet-state characterization. Theoritical analysis has been carried out to extend the use of FCS for photobleaching studies. In those analysis, triplet- and first excited singlet-state are considered as possible photobleaching pathways. Enviromental effects, such as singlet oxygen and external heavy-atom effects, were also discussed.