CORE--IMAGING

亨廷顿氏病（HD）是由新型亨廷顿蛋白中谷氨酰胺延伸的延长引起的。目前尚不清楚这种遗传缺陷如何导致HD的选择性病理。激光共聚焦免疫荧光光学显微镜已成为一个重要的工具，用于评估单或多个抗原的免疫荧光亚细胞定位。这种方法对于理解亨廷顿蛋白及其在HD细胞和组织中的突变对应物以及实验模型至关重要。然而，HD中心的研究人员对现有成像设施的使用受到限制，这造成了一个瓶颈，减缓了这些关键实验的进展。我们建议建立一个激光共聚焦成像核心设施，致力于支持HD中心研究人员的需求。 我们建议建立一个激光共聚焦成像核心设施，致力于支持HD中心研究人员的需求。新一代共焦显微镜技术的进步提供了同时采集多色图像，避免了渗色和减少了褪色。这一成像核心设施将大大提高HD中心研究人员获得高质量细胞生物学数据的效率和生产力。这些知识对于实现HD中心的长期目标至关重要，了解突变亨廷顿蛋白及其伙伴以及最终导致HD病理的正常和异常细胞功能。

Huntington's disease (HD) is caused by a elongation of a glutamine stretch in the novel huntingtin protein. It is not clear how this genetic defect leads to the selective pathology of HD. Laser confocal immunofluorescence light microscopy has become an essential tool for evaluating the subcellular localization of single or multiple antigens by immunofluorescence. This approach is essential to understanding huntingtin and its mutant counterpart in HD cells and tissues, and in experimental models . However, HD Center investigators have limited access to existing imaging facilities, creating a bottle neck that is slowing progress on these critical experiments. We propose to establish a laser confocal imaging core facility dedicated to supporting the needs of HD Center investigators. We propose to establish a laser confocal imaging core facility dedicated to supporting the needs of HD Center investigators. The advances in technology of the new generation of confocal microscopes provide for simultaneous acquisition of multi-color images free from bleed through and with reduced fading. This Imaging Core facility will greatly enhance the efficiency and productivity with which HD Center investigators can obtain high quality cell biological data. This knowledge is critical to achieving the long-term goal of the HD Center, understanding mutant huntingtin, its partners and normal and abnormal cellular functions that ultimately cause HD pathology.