CORE--TRANSGENIC/GENE DISRUPTION MOUSE

转基因/基因干扰小鼠核心研究的主要目标 是提供一个集中的面向服务的设施，它将 确保每个参与项目实验室的生产能力 转基因和基因破坏的小鼠。此外，这将导致 通过避免重复工作来节省工作量和费用 需要专门设备和专业技能的高技能 动物设施。转基因/基因中断的核心是 合作的主要途径。能够执行以下操作 体内的功能和丧失功能的实验将增强我们的 对引导呼吸道的分子机制的认识 炎症与哮喘的发病机制。此外， 跟踪组织和细胞类型表达的能力 启动子/增强子结合在正常胚胎和 成人的发展对加深我们对 呼吸道分化和炎症的调节。

The major objective of the Transgenic / Gene-Disruption Mouse Core is to provide a centralized service oriented facility that will insure the capacity of each participating project lab to produce transgenic and gene-disrupted mice. Moreover, this will result in an economy of effort as well as expense by avoiding duplication of a highly technical skill requiring specialized equipment and animal facilities. The Transgenic / Gene-Disruption Core is a major avenue for collaborations. The ability to perform gain of function and loss of function experiments in vivo will enhance our understanding of the molecular mechanisms guiding airway inflammation and the pathogenesis of asthma. Moreover, the capacity to follow the tissue and cell type expression of a Promoter/Enhancer combination throughout normal embryonic and adult development is crucial to furthering our understanding of airway differentiation and regulation of inflammation.