神经元分化的蛋白水解控制-猫眼课题宝

神经元分化的蛋白水解控制

批准号：

6337229

负责人：

James I Kim

金额：

$3.48万

依托单位：

UNIVERSITY OF CALIFORNIA LOS ANGELES

依托单位国家：

美国

项目类别：

财政年份：

2001

资助国家：

美国

项目状态：

未结题

起止时间：

2001-05-15 至

来源：

https://reporter.nih.gov/project-details/6337229

关键词：

cell differentiation gene deletion mutation gene expression gene targeting genetically modified animals in situ hybridization laboratory mouse molecular site neurogenesis northern blottings phenotype pluripotent stem cells postdoctoral investigator protease inhibitor proteolysis

中文摘要

翻译

我们已经发现了一种分泌的、可溶的蛋白质，它能够抑制非洲爪哇胚胎的初级神经发生。这个394个氨基酸的蛋白质含有三个kunitz类丝氨酸蛋白酶抑制域。这种新的蛋白质被命名为Trinein，并在对非洲爪哇卵中存在的母体编码的mRNAs进行差异筛选时分离出来。值得注意的是，将Trinein mRNA显微注射到非洲爪哇胚胎中，似乎对中胚层(以Xbra、音速刺猬、Chordin标记)和神经板(以SOX-2标记)的细胞分化几乎没有影响；然而，特定成熟神经元(神经微管蛋白和神经原蛋白阳性)的分化在神经板中被阻止(未发表的数据)。这一建议建立在Trinein的这些功能数据的基础上，以了解其在哺乳动物神经发生中的功能。

英文摘要

We have discovered a secreted, soluble protein that is able to inhibit primary neurogenesis in Xenopus embryos. This 394 amino acid protein contains three serine proteinase inhibitor domains of the Kunitz class. This novel protein has been named Trinein and was isolated during a differential screen for maternally-encoded mRNAs present in the Xenopus laevis egg. Strikingly, microinjection of Trinein mRNA into Xenopus embryos seems to have little effect on mesodermal (marked by Xbra, sonic hedgehog, chordin) and neural plate (marked by Sox-2) cell differentiation; however, differentiation of definitive mature neurons (positive for neurotubulin and neurogenin) is blocked in the neural plate (unpublished data). This proposal builds upon these functional data of Trinein to understand its function in mammalian neurogenesis.

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