TRANSPEPTIZYME INDUCED PEPTIDE BOND FORMATION

这项提议的长期目标是了解蛋白质合成的进化。具体来说，拟议的研究旨在建立基于trna受体干环的氨基酰基- rna系统，该系统将允许以非编码方式形成肽键。这种氨基酰基- rna复合物可能是一种原始的蛋白质翻译系统。RNA编码的遗传信息被翻译成蛋白质，而蛋白质是细胞中几乎所有生化和结构功能的核心。因此，分析多肽合成的基本过程可能有助于更好地了解人类疾病的起源。为实现上述目标，将采用两种策略：(1)利用基于P4-P6四膜核酶复合物结构的氨基酰化RA支架来构建肽；(2)开发由发夹微螺旋和单链核糖核苷酸组成的氨基酰化RNA三螺旋系统来生成肽。

The long-term objective of this proposal is to understand the evolution of protein synthesis. Specifically, the proposed research is designed to date aminoacyl-RNA systems based on the acceptor stem loop of tRNAs that will allow for peptide bond formation in a noncoded fashion. This aminoacyl-RNA complex may have served as a primitive protein translation system. The genetic information encoded by RNA is translated into proteins, which are central to virtually every biochemical and structural function in cells. Accordingly, analysis of the basic process of peptide synthesis may contribute to a greater understanding of the origins of human diseases. The two strategies that will be employed to achieve the proposed goal are 1) utilize an aminoacylatable RA scaffold based on the structure of the P4-P6 tetrahymena ribozyme complex to construct peptides and 2) develop an aminoacylated RNA triple helix system consisting of a hairpin minihelix and a single-stranded ribonucleotide to generate peptides.