Antioxidants, Oxidative Stress and Endurance Exercise

描述（由申请人提供） 人体运动产生的氧化应激和 通过抗氧化剂减轻其损害是该建议的基础。 的 研究人员先前已经证明，耐力运动会导致 在明显营养良好的运动员中脂质过氧化作用增加。 因此，他们计划使用相同的锻炼方案，即50公里的超级马拉松 作为模型系统运行，但将补充运行者 抗氧化剂或安慰剂。 要检验的假设是， 在耐力运动期间产生的氧化应激可以通过以下方式抑制： 抗氧化剂补充。 特别是补充维生素E 和/或维生素C，它们已知能抑制脂质过氧化， 有利于防止这种损害。 具体目标。 耐力运动的氧化应激是否可以通过 是否补充过抗氧化剂？ 32例受试者（16例女性，16例男性） 将在50公里（30英里）的超级马拉松比赛中进行研究。 受试者将 随机分配到四个研究组之一：（1）安慰剂组，（2）1000 mg 维生素C（500 mg，每日两次），（3）400 IU维生素E（RRR-alpha-生育酚 乙酸盐），或（4）1000 mg维生素C和400 IU维生素E两者。 受试者将 在比赛前6周内每天食用规定的饮食，比赛日， 整个抽血时间表。 受试者将服用规定的 比赛前6周每天节食，然后在比赛当天控制饮食 在比赛之前，受试者将消耗氘标记的维生素E（75 mg d 6 RRR-alpha-生育酚乙酸酯），其将作为维生素E的示踪剂 比赛中的失误。 血液样本将在术后3天内采集 用于测量d 6-α-生育酚、脂质标记物 过氧化和炎症标志物。 氘代生育酚消失 计算速率和曲线下面积（AUC）。 脂质过氧化 标志物（血浆F2-异前列腺素）、低密度脂蛋白（LDL）氧化 动力学和炎症标志物都将被测量。 拟议的研究将评估补充维生素E和/或 C可以降低耐力过程中产生的F2-异前列烷的水平 运动以及他们是否可以降低氘代维生素E 消失. 最后，研究人员计划测量 炎症反应，如C-氧化剂增加和维生素E减少 这些标记。

DESCRIPTION (provided by applicant) Oxidative stress generated by exercising humans and the potential for attenuating its damage by antioxidants is the basis for this proposal. The investigators have demonstrated previously that endurance exercise causes an increase in lipid peroxidation in apparently well nourished athletes. Therefore, they plan to use the same exercise protocol, a 50 km ultramarathon run as the model system with the exception that runners will be supplemented with antioxidants or placebo. The hypothesis to be tested is that the oxidative stress generated during endurance exercise can be inhibited by antioxidant supplementation. Specifically, supplementation with vitamin E and/or vitamin C, which are known to inhibit lipid peroxidation, could be beneficial in preventing such damage. Specific Aim. Can the oxidative stress of endurance exercise be alleviated by prior antioxidant supplementation? Thirty-two subjects (16 females, 16 males) will be studied during a 50 km (30 mile) ultramarathon. Subjects will be randomly assigned to one of four study groups: (1) placebos, (2) 1000 mg vitamin C (500 mg twice a day), (3) 400 IU vitamin E (RRR-alpha-tocopheryl acetate), or (4) both 1000 mg vitamin C and 400 IU vitamin E. Subjects will consume a prescribed diet daily for 6 weeks prior to the race, race day and throughout the blood drawing schedule. Subjects will consume a prescribed diet daily for 6 weeks prior to the race, and then a controlled diet the day prior to the race, subjects will consume deuterium-labeled vitamin E (75 mg d6RRR-alpha-tocopheryl acetate) that will act as a tracer for vitamin E turnover during the race. Blood samples will be obtained up to 3 days after the race for measurements of d6-alpha-tocopherol, markers of lipid peroxidation and markers of inflammation. Deuterated tocopherol disappearance rates and areas under the curve (AUC) will be calculated. Lipid peroxidation markers (plasma F2-isoprostanes), low density lipoprotein (LDL) oxidation kinetics, and markers of inflammation will all be measured. The proposed study will evaluate whether supplementation with vitamin E and/or C can decrease the levels of F2-isoprostanes generated during endurance exercise and whether they can reduce the rate at which deuterated vitamin E disappears. Finally, the investigators plan to measure markers of inflammatory response, such as C-oxidants increase and vitamin E decreases these markers.