Genetics of Anopheles funestus populations

描述(申请人提供)：连同冈比亚按蚊和冈比亚按蚊 一种。Arabiens，An.Funestus是世界上最重要的疟疾媒介之一 世界。在非洲部分地区，子孢子虫率高达9%， 清除这一点。在任何全面的控制计划中都不能忽视Funestus 或者从这块大陆根除疟疾。无论控制策略是什么 针对AN实施的。冈比亚亚种必须与控制相结合，以防止 一种。福斯特斯。因为目前不可能维持An的群体。 Funestus在实验室中，无论是经典的基因控制方案还是 转基因策略是可以开发的。然而，一个详细的重要性 对媒介种群结构的理解超越了遗传控制 计划，因为这方面的知识对成功使用 在财力和人力资源有限的情况下，在病媒控制中使用杀虫剂。 这一知识使我们能够对矢量的响应做出预测 人口对人类干预或基因变化的影响，例如， 导致杀虫剂抗药性的突变的传播。不幸的是， 按蚊的群体遗传学结构。人们对Funestus知之甚少， 迄今为止，这方面的研究相对较少。很少有分子遗传标记被 为这个物种开发的。这项提案中概述的实验将 使用我们开发的细胞遗传学作图的分子标记 解决一个国家内部和两个国家之间基因流动的基本问题。福斯特斯 人口。底座的大小是多少，距离和距离的影响是什么 德梅斯人之间基因流动的栖息地？携带的是另一种染色体 反转排列是繁殖隔离的？他们有没有流行病学上的 咬人和休息行为的相关差异？从以下方面获得的信息 这些标记的使用将使我们能够更有效地管理 目前可用的媒介控制工具，并将奠定必要的 基金会If Gentis针对An的策略。Funestus成为可能

DESCRIPTION (provided by the applicant): Together with Anopheles gambiae and An. arabiensis, An. funestus is among the most important malaria vectors in the world. With sporozoite rates as high as 9 percent in parts of Africa, it is clear that An. funestus cannot be ignored in any comprehensive plan to control or eradicate malaria from this continent. Whatever control strategy is implemented against An. gambiae s.l. must be integrated with control against An. funestus. Because it is currently impossible to maintain colonies of An. funestus in the laboratory, neither classical genetic control schemes nor transgenic strategies can be developed. However, the importance of a detailed understanding of vector population structure transcends genetic control programs, as this knowledge is also pivotal to the successful use of insecticides in vector control, given limited financial and human resources. This knowledge allows predictions to be made about the response of vector populations to human interventions or genetic changes, e.g., the potential for spread of mutations conferring insecticide resistance. Unfortunately, the population genetics structure of An. funestus is poorly understood and relatively unstudied to date. Very few molecular genetic markers have been developed for this species. The experiments outlined in this proposal will employ cytogenetically mapped molecular markers that we have developed to address fundamental questions about gene flow within and between An. funestus populations. What is the size of a deme and what is the effect of distance and habitat on gene flow between demes? Are carries of alternative chromosomal inversion arrangements reproductively isolated? Do they have epidemiologically relevant differences in biting and resting behavior? Information gained from the use of these markers will enable us to more efficiently manage the currently available tools for vector control and will lay the necessary foundation if genetis strategies against An. funestus become possible