TRYPANOSOMA CRUZI-INDUCED HOST CELL SIGNALING RESPONSES

描述(改编自申请人的摘要)：信号转导 哺乳动物感染的克鲁兹毛滴虫在宿主细胞中触发的途径 是入侵过程所必需的。特别是第二信使Ca+2 和cAMP是克氏毛滴虫诱导宿主细胞信号转导的已知效应器。目标 这一建议的目的是通过 确定所涉及的特定宿主蛋白因子。寄主 细胞激酶和磷酸酶将被识别及其在侵袭中的作用 和/或宿主细胞基因表达的特征。由以下因素引发的事件 野生型克氏锥虫将与由突变株诱导的克氏锥虫进行比较。 在触发特定的Ca+2宿主细胞反应方面存在缺陷，这是 哺乳动物细胞的入侵。最后，将进行基因芯片杂交 用来识别在T。 克鲁兹病毒感染。

DESCRIPTION (Adapted from the Applicant's Abstract): Signal transduction pathways triggered in host cells by the mammalian-infective forms of T. cruzi are required for the invasion process. Specifically the second messengers Ca+2 and cAMP are known effectors of T. cruzi induced host cell signaling. The goals of this proposal are to elucidate these signaling pathways by the identification of the specific host protein factors that are involved. Host cell kinases and phosphatases will be identified and their roles in invasion and/or host cell gene expression will be characterized. The events induced by wild-type T. cruzi will be compared to those induced by a mutant strain that is defective in triggering a specific Ca+2 host cell response, required for invasion of mammalian cells. Finally, cDNA micro array hybridization will be used to identify host cell genes that are differentially expressed during T. cruzi infection.