Skin penetration and metabolism of a new micellar retinol formulation for cosmetics and therapeutics

本研究的目的是了解phytoceeutical的纳米胶束视黄醇配方是如何工作的，以及它们在使用后对皮肤的影响。为了做到这一点，我们将使用一种特殊类型的模型来模拟皮肤的不同层。在应用植物制剂之前，该模型将暴露在紫外线（UV）光下以模拟太阳照射。在研究中，我们将测试三种不同浓度的植物醇制剂（0.1%，0.3%和1%），并将结果与标准视黄醇产品和不使用产品的对照组进行比较。为了观察产品对皮肤模型的影响，我们将使用两种专用显微镜：薄层显微镜和多光子显微镜。使用薄片显微镜，我们将检查产品如何在皮肤模型中扩散，以及它如何影响皮肤细胞的形状和位置。通过多光子显微镜，我们将看到该产品对胶原蛋白生产的影响，胶原蛋白是一种重要的蛋白质，有助于保持皮肤年轻，以及皮肤伤口的愈合。我们还将使用两种不同的光谱技术来跟踪视黄醇到视黄酸的转化，这是一个发生在皮肤中的过程。这将为我们提供有关植物制剂是否影响这一过程的信息以及它们与标准视黄醇产品和对照组相比如何。通过比较植物制剂、标准视黄醇产品和对照组的结果，我们将能够确定用于护肤产品的植物制剂的最佳浓度。这些信息对于开发安全有效的产品非常重要，这些产品可以帮助人们获得更健康、更年轻的皮肤。

The purpose of this study is to find out how Phytoceutical's nano-micellar retinol formulations work and how they impact the skin after use. To do this, we will use a special type of model that simulates the different layers of skin. This model will be exposed to ultraviolet (UV) light to simulate sun exposure before the Phyto formulations are applied.In the study, we will test three different concentrations of Phyto formulations (0.1%, 0.3%, and 1%) and compare the results to a standard retinol product and a control group where no product is applied.To observe the effects of the products on the skin model, we will use two types of special microscopes: light sheet microscopes and multiphoton microscopes. With the light sheet microscopes, we will examine how the product spreads in the skin model and how it affects the shape and position of the skin cells. With the multiphoton microscopes, we will see the effects of the product on the production of collagen, which is an important protein that helps keep skin looking youthful, and on the healing of wounds in the skin.We will also use two different spectroscopy techniques to track the conversion of retinol to retinoic acid, which is a process that occurs in the skin. This will give us information about whether the Phyto formulations are affecting this process and how they compare to the standard retinol product and the control group.By comparing the results of the Phyto formulations, the standard retinol product, and the control group, we will be able to determine the optimal concentration of Phyto formulations for use in skin care products. This information will be important for developing safe and effective products that can help people achieve healthier, more youthful-looking skin.