THE ROLE OF LFA-1 IN T CELL ACTIVATION

描述（改编自应用程序） Clara Abraham，医学博士，获得了计算机科学学士学位， 1985年在伊利诺伊大学获得工程学院的高级荣誉， 1989年在普利兹克的芝加哥大学获得医学学位 医学院。在拉什的内科住院医师之后 长老会-圣卢克医疗中心，她完成了三年的基本 芝加哥大学胃肠病学科学奖学金。期间 在这期间，她进入了托马斯博士的实验室。Brasitus，并研究了 蛋白激酶C和1，25（OH）2D 3在细胞生长、分化和增殖中的作用 Caco-2人结肠癌细胞的凋亡。继三年 奖学金，她进入实验室博士吉姆米勒，以追求 基础科学免疫学的额外培训。她一直在调查 LFA-Ⅰ在T淋巴细胞活化中的作用。 在目前的建议中，亚伯拉罕博士计划继续探讨有关在 LFA-1在T细胞功能中的体外和体内作用。具体来说，她打算 进一步了解和分离的具体功能的贡献， LFA-I通过体外诱变在T细胞活化中的作用。接着她会 利用体外获得的信息来识别特异性的、显著的T细胞， 待在体内炎症模型中分析的表型，特别是， 炎症性肠病的鼠模型。这种方法的目标是 允许LFA-1对T细胞运输的贡献相对于T细胞运输的贡献的分离。 细胞活化，并评估这些功能在体内的相对重要性。 由于Abraham博士没有接受过IBD和肠道疾病动物模型的正式培训， 免疫学，拟议的研究职业发展计划要求支持 五年，以提供一个正式的，全面的培训计划， 这将使她能够建立一个独立的实验室， 研究这些体内炎症模型。

DESCRIPTION (adapted from the application) Clara Abraham, MD, received a baccalaureate degree in computer science from the College of Engineering, high honors, in 1985 at the University of Illinois, Urbana, and a medical degree in 1989 at the University of Chicago, Pritzker School of Medicine. Following internal medicine residency at Rush Presbyterian-St. Luke's Medical Center, she completed a three year basic science fellowship in gastroenterology at The University of Chicago. During this period, she entered the laboratory of Dr. Thomas A. Brasitus, and studied the role of protein kinase C and 1,25(OH)2D3 in the growth, differentiation and apoptosis of Caco-2 human colon cancer cells. Following the three year fellowship, she entered the laboratory of Dr. Jim Miller in order to pursue additional training in basic science immunology. She has been investigating the role of LFA- I in the activation of T lymphocytes for the past 3 years. In the present proposal, Dr. Abraham plans to pursue questions regarding the in vitro and in vivo role of LFA-1 in T cell function. Specifically, she intends to further understand and segregate the contributions of specific functions of LFA-I within T cell activation through in vitro mutagenesis. She will then utilize the information acquired in vitro to identify specific, dramatic T cell phenotypes to be analyzed in in vivo models of inflammation, in particular, murine models of inflammatory bowel disease. The goal of such an approach is to allow for segregation of LFA-l's contribution to T cell trafficking versus T cell activation, and assess the relative importance of these functions in vivo. As Dr. Abraham has no formal training in animal models of IBD and intestinal immunology, the proposed research career development plan requests support for five years in order to provide a formalized, comprehensive training program for her that will allow her to establish an independent laboratory to pursue research in these in vivo models of inflammation.