One Step H. pylori Test--Screening and Individual Assays

该项目的目的是证明四种不同类型的H。幽门螺杆菌测定。将对H.幽门螺杆菌及其抗体。具体目标将是开发一种快速和成本极低的检测方法，用于人群筛查和临床实验室。H.幽门螺杆菌是一种重要的癌症风险，因为它可以导致两种类型的恶性肿瘤：胃腺癌和原发性胃MALT淋巴瘤。胃腺癌是世界范围内癌症死亡的第二大原因，预计到2010年将成为所有死亡的第八大原因。所提出的方法是表面增强拉曼免疫分析（SERIA），具有几个显着的优点。最重要的是，SERIA方法的表面局部增强消除了来自基质效应的光谱干扰。此外，它消除了在其他免疫测定配置中产生生物危害废物和人员暴露的洗涤步骤的需要。该项目将研究敏感性，选择性，保质期，和生产成本的H。幽门螺杆菌SERIA。将使用无菌缓冲液优化SERIA。一旦用缓冲液建立了典型的方案，我们将检查以小鼠组织、血液、粪便和胃液形式提供的动物样品。当动物样本的优化建立后，我们将研究人血。组织粪便和呕吐物样本将与市售试剂盒进行比较。建议的商业应用：该项目的首要目标是生产一种微孔格式的H。幽门。第二个目标是小批量测试的单一样本格式第一个目标的市场是开放的，因为没有大批量低成本的程序存在。市场将是用于筛查大量幽门螺杆菌感染的仪器和检测。

The aim of this project is to demonstrate feasibility of four different types of H. pylori assays. Assays will be developed for both the H. pylori organism and its antibody. The specific goal will be to develop a test which is rapid and very-low cost for screening of populations and for clinical laboratories. H. pylori is significant as a cancer risk as it can cause two types of malignancies: gastric adenocarcinoma and primary - gastric MALT lymphoma. The gastric adenocarcinoma is the second leading cause of cancer death around the world and it is expected by 2010 to be the eighth leading cause of all death. The method proposed is a Surface Enhanced Raman ImmunoAssay (SERIA) that has several significant advantages. Foremost, the surface localized enhancement of the SERIA method eliminates spectroscopic interference from matrix effects. Furthermore, it eliminates the need for washing steps that create biohazardous waste and personnel exposure in other immunoassays configurations. The project will examine sensitivity, selectivity, shelf-- life, and cost of production for H. pylori SERIA. The SERIA will be optimized with sterile buffer solutions. Once typical protocols are established with buffers we will examine animal samples supplied in the for of mice tissue, blood, feces, and gastric juices. When optimization with the animals samples has been established we will look at human blood. tissue, feces, and vomitus samples. Comparisons will made to commercially available kits. PROPOSED COMMERCIAL APPLICATIONS: The number one goal of this project is to produce a microwell format assay for H. pylori. Secondary is a single sample format for low-volume testing The market for the first goal is wide open as no high-volume low-cost procedure exists. The market would be instruments and assays for screening large populations for H pylori infections.