Output of the Circadian Clock

描述（由申请人提供）：昼夜（约24小时）节律， 受生物钟控制的生物钟存在于许多人的生理和行为中， 包括人类和果蝇在内的生物。在哺乳动物中， 最明显的昼夜节律的例子， 对健康的影响，但对体温、心跳、血压的调节， 内分泌功能、肾活动和肝脏代谢也已显示 处于昼夜节律调节之下生物钟产生振荡 控制生物钟自身节奏的转录模式 以及他们的产出。本研究项目的目标是 第一次在全基因组范围内进行测试， 果蝇受生物钟的调节，然后确定一个子集， 这些转录本参与调节休息活动， 周期DNA微阵列将用于进行表达分析; 自发活动测定将作为表型测量， 休息-活动周期;遗传和分子技术的结合将 在候选监管机构的功能分析， 休息-活动周期时钟控制的综合描述 果蝇头部的转录本将为未来所有的研究提供宝贵的资源。 研究昼夜节律，而新的介质的发现， 休息-活动周期将促进对分子的更好理解， fruitfiles中的休息-活动周期和 类似哺乳动物的睡眠-觉醒周期。

DESCRIPTION (provided by applicant): Circadian (-24 hour) rhythms that are controlled by circadian clocks are found in the physiology and behavior of many organisms including humans and fruitflies. In mammals the sleep-wake cycle is the most obvious example of a circadian rhythm that can have important effects on health, but the regulation of body temperature, heartbeat, blood pressure, endocrine functions, renal activity, and liver metabolism have also been shown to be under circadian regulation. Circadian clocks generate oscillating patterns of transcription that control both the rhythm of the clocks themselves and the output that they produce. It is the goal of this research project to first test on a genome-wide scale which transcripts in the head of adult fruitflies are regulated by circadian clocks and then identify a subset of these transcripts that are involved in the regulation of the rest-activity cycle. DNA microarrays will be used to perform the expression analyses; locomotor activity assays will serve as a phenotypic measure for the rest-activity cycle; and a combination of genetic and molecular techniques will be employed in the functional analysis of candidate regulators of the rest-activity cycle. The comprehensive description of the clock controlled transcripts in Drosophila heads will provide a valuable resource for all future studies on circadian rhythms, whereas the discovery of new mediators of the rest-activity cycle will promote a better understanding of the molecular mechanisms underlying both the rest-activity cycle in fruitfiles and the analogous sleep-wake cycle in mammals.