RNA TRANSPORT IN DENDRITES

描述（根据申请人摘要修改）在神经细胞中，调节 一些关键的突触后蛋白在树突中的局部合成可以 对长期的结构和功能至关重要， 突触连接的可塑性这样一个重要的先决条件是， 其机制是将选择的RNA靶向转运到树突状位点， 翻译.树突中特异性RNA转运的概念已经获得 最近在申请人的实验室中观察到顺式作用的 树突状RNA中包含的信号元件负责 用于将RNA靶向树突。根据这些数据， 本研究项目的中心目标被定义为解剖 树突中RNA转运机制的研究及反馈分析 能够局部调节树突状RNA的机制 通过突触处的生理事件进行运输。具体目标1 计划在初级和/或更高级方面定义 结构，最小必要目标的物理精细结构 模块。在第二个目标中，将确定是否以及如何 树突状RNA的转运通过神经元活性调节。下 这个具体目标是什么机制正在使用的问题， 神经元调节树突蛋白的多样性， 突触的功能性输入本研究项目的总体目标是 因此，确定树突状RNA的功能意义， 运输机制，可能是至关重要的， 调节镶嵌突触后蛋白库，从而为 神经间突触连接的发育和长期可塑性 细胞

DESCRIPTION (Modified from applicant's abstract) In nerve cells, regulated local synthesis in dendrites of a number of key postsynaptic proteins may be of critical relevance for the long-term structural and functional plasticity of synaptic connections. An essential prerequisite for such a mechanism is the targeted transport of select RNAs to dendritic sites of translation. The notion of specific RNA transport in dendrites has gained support by the recent observation in the applicant's lab that a cis-acting signaling element that is contained with a dendritic RNA is responsible for the targeting of the RNA to dendrites. Based on these data, the central objectives of this research project are defined as the dissection of RNA transport mechanisms in dendrites and the analysis of feedback mechanisms that would allow the local regulation of dendritic RNA transport through physiological events at the synapse. In Specific Aim 1 it is planned to define, in terms of primary and/or higher order structure, the physical fine structure of minimal essential targeting module(s). In the Second Aim, it will be established whether and how transport of dendritic RNAs is regulated through neuronal activity. Under this Specific Aim is the question of what mechanisms are being used by neurons to modulate the diversity of dendritic proteins in response to functional input at the synapse. The overall goal of this research project is thus to ascertain the functional significance of dendritic RNA transport mechanisms that are likely to be of vital importance in the regulation of mosaic postsynaptic protein repertoires, and thus for the development and long-term plasticity of synaptic connections between nerve cells.